• Product name
    Anti-53BP1 (phospho S25) antibody
    See all 53BP1 primary antibodies
  • Description
    Rabbit polyclonal to 53BP1 (phospho S25)
  • Host species
  • Tested applications
    Suitable for: IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Baboon, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Phosphorylated synthetic peptide corresponding to a portion of 53BP1 around serine 25 (NP_005648.1)

  • Positive control
    • Human colon adenocarcinoma tissue.



Our Abpromise guarantee covers the use of ab82550 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Function
    May have a role in checkpoint signaling during mitosis. Enhances TP53-mediated transcriptional activation. Plays a role in the response to DNA damage.
  • Involvement in disease
    Note=A chromosomal aberration involving TP53BP1 is found in a form of myeloproliferative disorder chronic with eosinophilia. Translocation t(5;15)(q33;q22) with PDGFRB creating a TP53BP1-PDGFRB fusion protein.
  • Sequence similarities
    Contains 2 BRCT domains.
  • Post-translational
    Asymmetrically dimethylated on Arg residues by PRMT1. Methylation is required for DNA binding.
    Phosphorylated at basal level in the absence of DNA damage. Hyper-phosphorylated in an ATM-dependent manner in response to DNA damage induced by ionizing radiation. Hyper-phosphorylated in an ATR-dependent manner in response to DNA damage induced by UV irradiation.
  • Cellular localization
    Nucleus. Chromosome > centromere > kinetochore. Associated with kinetochores. Both nuclear and cytoplasmic in some cells. Recruited to sites of DNA damage, such as double stand breaks. Methylation of histone H4 at 'Lys-20' is required for efficient localization to double strand breaks.
  • Information by UniProt
  • Database links
  • Alternative names
    • 53 BP1 antibody
    • 53BP1 antibody
    • FLJ41424 antibody
    • MGC138366 antibody
    • p202 antibody
    • p53 binding protein 1 antibody
    • p53 BP1 antibody
    • p53-binding protein 1 antibody
    • p53BP1 antibody
    • TP53 BP1 antibody
    • TP53B_HUMAN antibody
    • Tp53bp1 antibody
    • TRP53 BP1 antibody
    • Tumor protein 53 binding protein 1 antibody
    • Tumor protein p53 binding protein 1 antibody
    • Tumor suppressor p53 binding protein 1 antibody
    • Tumor suppressor p53-binding protein 1 antibody
    see all


  • ab82550, at a 1:250 dilution, staining Human 53BP1 (phospho Ser25) in colon adenocarcinoma (mock phosphatase treated section), using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.
  • ab82550, at a 1:250 dilution, staining Human 53BP1 (phospho Ser25) in colon adenocarcinoma (lambda and CIP phosphatase treated), using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue,


This product has been referenced in:
  • Lunardi A  et al. Suppression of CHK1 by ETS Family Members Promotes DNA Damage Response Bypass and Tumorigenesis. Cancer Discov 5:550-63 (2015). IHC ; Human . Read more (PubMed: 25653093) »
  • Roossink F  et al. The role of ATM and 53BP1 as predictive markers in cervical cancer. Int J Cancer 131:2056-66 (2012). IHC-P ; Human . Read more (PubMed: 22323184) »

See all 2 Publications for this product

Customer reviews and Q&As

These are very similar antibodies.

The ab82550 Anti-53BP1 (phospho S25) antibody is reformulated to specifically cater to needs expressed by IHC specialists to have antibodies that can be used between a 1:100 and 1:500 dilution. Both are teste...

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