Overview

  • Product name

    Anti-53BP2/ASPP2/BBP antibody
    See all 53BP2/ASPP2/BBP primary antibodies
  • Description

    Rabbit polyclonal to 53BP2/ASPP2/BBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human 53BP2/ASPP2/BBP (N terminal). The exact sequence is proprietary.
    Database link: Q13625

  • Positive control

    • WB: U-2 OS, mouse MEF and INS-1 cell lysates. IP: U-2 OS cell lysate. ICC/IF: U-2 OS cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP.
  • General notes

     This product was previously labelled as 53BP2/ASPP2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.05% Sodium azide
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab236448 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 126 kDa.
ICC/IF 1/2000.
IP Use at an assay dependent concentration.

Use 3 µl to ~400 µg of extact

Target

  • Function

    Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
  • Tissue specificity

    Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
  • Sequence similarities

    Belongs to the ASPP family.
    Contains 2 ANK repeats.
    Contains 1 SH3 domain.
  • Domain

    The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.
  • Cellular localization

    Cytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
  • Information by UniProt
  • Database links

  • Alternative names

    • 53BP2 antibody
    • Apoptosis stimulating of p53 protein 2 antibody
    • Apoptosis stimulating protein of p53 2 antibody
    • Apoptosis-stimulating of p53 protein 2 antibody
    • ASPP2 antibody
    • ASPP2_HUMAN antibody
    • BBP antibody
    • Bcl2 binding protein antibody
    • Bcl2-binding protein antibody
    • NY REN 51 antigen antibody
    • p53 binding protein 2 antibody
    • p53-binding protein 2 antibody
    • p53BP2 antibody
    • PPP1R13A antibody
    • Renal carcinoma antigen NY-REN-51 antibody
    • Tp53bp2 antibody
    • Tumor protein p53 binding protein 2 antibody
    • Tumor suppressor p53 binding protein 2 antibody
    • Tumor suppressor p53-binding protein 2 antibody
    see all

Images

  • All lanes : Anti-53BP2/ASPP2/BBP antibody (ab236448) at 1/2000 dilution

    Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with control siRNA
    Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with 53BP2/ASPP2/BBP siRNA
    Lane 3 : Mouse MEF cell lysate
    Lane 4 : Rat INS-1 cell lysate

    Predicted band size: 126 kDa

  • 2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP stained for 53BP2/ASPP2/BBP (red) using ab236448 at 1/2000 dilution in ICC/IF. GFP (green). Right panel shows the merged images.

  • 2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP stained for 53BP2/ASPP2/BBP (red) using ab236448 at 1/2000 dilution in ICC/IF. GFP (green). Lower panel shows the merged images.

  • 53BP2/ASPP2/BBP was immunoprecipitated from ~400 µg U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate with with 3 μl ab236448. Western blot was performed from the immunoprecipitate using ab236448 at 1/2000 dilution.

    Lane 1: U-2 OS cell lysate.

    Lane 2: Control IP in U-2 OS cell lysate.

    Lane 3: ab236448 IP in U-2 OS cell lysate.

References

ab236448 has not yet been referenced specifically in any publications.

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