Key features and details
- Rabbit polyclonal to 53BP2/ASPP2/BBP
- Suitable for: WB, ICC/IF, IP
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-53BP2/ASPP2/BBP antibody
See all 53BP2/ASPP2/BBP primary antibodies
DescriptionRabbit polyclonal to 53BP2/ASPP2/BBP
Tested applicationsSuitable for: WB, ICC/IF, IPmore details
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment within Human 53BP2/ASPP2/BBP (N terminal). The exact sequence is proprietary.
Database link: Q13625
- WB: U-2 OS, mouse MEF and INS-1 cell lysates. IP: U-2 OS cell lysate. ICC/IF: U-2 OS cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP.
This product was previously labelled as 53BP2/ASPP2
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Concentration information loading...
Our Abpromise guarantee covers the use of ab236448 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000. Predicted molecular weight: 126 kDa.|
|IP||Use at an assay dependent concentration.
Use 3 µl to ~400 µg of extact
FunctionRegulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
Tissue specificityWidely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
Sequence similaritiesBelongs to the ASPP family.
Contains 2 ANK repeats.
Contains 1 SH3 domain.
DomainThe ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.
Cellular localizationCytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
- Information by UniProt
- 53BP2 antibody
- Apoptosis stimulating of p53 protein 2 antibody
- Apoptosis stimulating protein of p53 2 antibody
All lanes : Anti-53BP2/ASPP2/BBP antibody (ab236448) at 1/2000 dilution
Lane 1 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with control siRNA
Lane 2 : U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate treated with 53BP2/ASPP2/BBP siRNA
Lane 3 : Mouse MEF cell lysate
Lane 4 : Rat INS-1 cell lysate
Predicted band size: 126 kDa
2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP stained for 53BP2/ASPP2/BBP (red) using ab236448 at 1/2000 dilution in ICC/IF. GFP (green). Right panel shows the merged images.
2% paraformaldehyde-fixed U-2 OS (human bone osteosarcoma epithelial cell line) cells transfected with a plasmid expressing 53BP2/ASPP2/BBP GFP stained for 53BP2/ASPP2/BBP (red) using ab236448 at 1/2000 dilution in ICC/IF. GFP (green). Lower panel shows the merged images.
53BP2/ASPP2/BBP was immunoprecipitated from ~400 µg U-2 OS (human bone osteosarcoma epithelial cell line) cell lysate with with 3 μl ab236448. Western blot was performed from the immunoprecipitate using ab236448 at 1/2000 dilution.
Lane 1: U-2 OS cell lysate.
Lane 2: Control IP in U-2 OS cell lysate.
Lane 3: ab236448 IP in U-2 OS cell lysate.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab236448 has not yet been referenced specifically in any publications.