Product nameAnti-53BP2/ASPP2/BBP antibody
See all 53BP2/ASPP2/BBP primary antibodies
DescriptionRabbit polyclonal to 53BP2/ASPP2/BBP
Tested applicationsSuitable for: IP, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Synthetic peptide aa 825-875. The exact sequence is proprietary. NP_001026855.1
Database link: Q13625
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate. IHC-P: Human ovarian carcinoma and mouse squamous cell carcinoma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab264151 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/1000 - 1/5000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
|WB||1/2000 - 1/10000. Predicted molecular weight: 126 kDa.|
FunctionRegulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
Tissue specificityWidely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
Sequence similaritiesBelongs to the ASPP family.
Contains 2 ANK repeats.
Contains 1 SH3 domain.
DomainThe ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.
Cellular localizationCytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
- Information by UniProt
- 53BP2 antibody
- Apoptosis stimulating of p53 protein 2 antibody
- Apoptosis stimulating protein of p53 2 antibody
All lanes : Anti-53BP2/ASPP2/BBP antibody (ab264151) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T whole cell lysate at 50 µg
Predicted band size: 126 kDa
Exposure time: 30 seconds
53BP2/ASPP2/BBP was immunoprecipitated from 1mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab264151 at 3 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab264151 at 1 µg/ml.
Lane 1: ab264151 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Exposure time: 10 secs.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human ovarian carcinoma (left panel) and mouse squamous cell carcinoma (right panel) labeling 53BP2/ASPP2/BBP with ab264151 at 1/1,000 dilution. Detection: DAB.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab264151 has not yet been referenced specifically in any publications.