• Product name
    Anti-53BP2/ASPP2/BBP antibody
    See all 53BP2/ASPP2/BBP primary antibodies
  • Description
    Rabbit polyclonal to 53BP2/ASPP2/BBP
  • Host species
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human 53BP2/ASPP2/BBP.
    Database link: NP_001026855.1

  • Positive control
    • Whole cell lysate from HeLa or 293T cells
  • General notes

     This product was previously labelled as 53BP2/ASPP2



  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium azide
    Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab70548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Detects a band of approximately 170 kDa (predicted molecular weight: 125 kDa).
IP Use at 2-5 µg/mg of lysate.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.


  • Function
    Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
  • Tissue specificity
    Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
  • Sequence similarities
    Belongs to the ASPP family.
    Contains 2 ANK repeats.
    Contains 1 SH3 domain.
  • Domain
    The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.
  • Cellular localization
    Cytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
  • Information by UniProt
  • Database links
  • Alternative names
    • 53BP2 antibody
    • Apoptosis stimulating of p53 protein 2 antibody
    • Apoptosis stimulating protein of p53 2 antibody
    • Apoptosis-stimulating of p53 protein 2 antibody
    • ASPP2 antibody
    • ASPP2_HUMAN antibody
    • BBP antibody
    • Bcl2 binding protein antibody
    • Bcl2-binding protein antibody
    • NY REN 51 antigen antibody
    • p53 binding protein 2 antibody
    • p53-binding protein 2 antibody
    • p53BP2 antibody
    • PPP1R13A antibody
    • Renal carcinoma antigen NY-REN-51 antibody
    • Tp53bp2 antibody
    • Tumor protein p53 binding protein 2 antibody
    • Tumor suppressor p53 binding protein 2 antibody
    • Tumor suppressor p53-binding protein 2 antibody
    see all


  • IHC image of ab70548 staining in normal human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab70548, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-53BP2/ASPP2/BBP antibody (ab70548) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg
    Lane 4 : Whole cell lysate from 293T cells at 50 µg

    Developed using the ECL technique.

    Predicted band size: 125 kDa
    Observed band size: 170 kDa
    why is the actual band size different from the predicted?

    Exposure time: 30 seconds
  • 1mg whole cell lysate from HeLa cells was immunoprecipitated with ab70548 at 3ug/mg of lysate(lane 1) or a control IgG (lane 2). Fr the subsequent blot, 20% of immnunoprecipitate was loaded per lane, and probed with ab70548 at 1ug/ml. Detection: chemiluminescence with exposure time of 30 seconds


ab70548 has not yet been referenced specifically in any publications.

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