Overview

  • Product name

    Anti-53BP2/ASPP2/BBP antibody
    See all 53BP2/ASPP2/BBP primary antibodies
  • Description

    Rabbit polyclonal to 53BP2/ASPP2/BBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human 53BP2/ASPP2/BBP aa 825-875.
    Database link: NP_001026855.1

  • Positive control

    • Human prostate adenocarcinoma and mouse squamous cell carcinoma tissues.
  • General notes

     This product was previously labelled as 53BP2/ASPP2

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab84996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
  • Application notes
    IHC-P: 1/100 - 1/500.
    Epitope exposure with citrate buffer will enhance staining.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
    • Tissue specificity

      Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
    • Sequence similarities

      Belongs to the ASPP family.
      Contains 2 ANK repeats.
      Contains 1 SH3 domain.
    • Domain

      The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.
    • Cellular localization

      Cytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
    • Information by UniProt
    • Database links

    • Alternative names

      • 53BP2 antibody
      • Apoptosis stimulating of p53 protein 2 antibody
      • Apoptosis stimulating protein of p53 2 antibody
      • Apoptosis-stimulating of p53 protein 2 antibody
      • ASPP2 antibody
      • ASPP2_HUMAN antibody
      • BBP antibody
      • Bcl2 binding protein antibody
      • Bcl2-binding protein antibody
      • NY REN 51 antigen antibody
      • p53 binding protein 2 antibody
      • p53-binding protein 2 antibody
      • p53BP2 antibody
      • PPP1R13A antibody
      • Renal carcinoma antigen NY-REN-51 antibody
      • Tp53bp2 antibody
      • Tumor protein p53 binding protein 2 antibody
      • Tumor suppressor p53 binding protein 2 antibody
      • Tumor suppressor p53-binding protein 2 antibody
      see all

    Images

    • ab84996 at 1/500 dilution staining 53BP2/ASPP2/BBP in human prostate adenocarcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

    • ab84996 at 1/100 dilution staining 53BP2/ASPP2/BBP in mouse squamous cell carcinoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.

    References

    ab84996 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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