Overview

  • Product name

    Anti-53BP2/ASPP2/BBP antibody [EPR13837]
    See all 53BP2/ASPP2/BBP primary antibodies
  • Description

    Rabbit monoclonal [EPR13837] to 53BP2/ASPP2/BBP
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: ICC/IF
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human 53BP2/ASPP2/BBP aa 50-150. The exact sequence is proprietary.
    Database link: Q13625

  • Positive control

    • WB: Rat and mouse brain lysates; MCF-7, Jurkat and Saos-2 cell lysates IHC-P: Human kidney, spleen and testis tissue; Rat and mouse liver tissue
  • General notes

    This product was previously labelled as 53BP2/ASPP2

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR13837
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab181377 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/10000 - 1/50000. Detects a band of approximately 150 kDa (predicted molecular weight: 126 kDa).
IHC-P 1/1000.

See IHC antigen retrieval protocols.

For unpurified use at 1/100 - 1/250.

  • Application notes
    Is unsuitable for ICC/IF.
  • Target

    • Function

      Regulator that plays a central role in regulation of apoptosis and cell growth via its interactions. Regulates TP53 by enhancing the DNA binding and transactivation function of TP53 on the promoters of proapoptotic genes in vivo. Inhibits the ability of APPBP1 to conjugate NEDD8 to CUL1, and thereby decreases APPBP1 ability to induce apoptosis. Impedes cell cycle progression at G2/M. Its apoptosis-stimulating activity is inhibited by its interaction with DDX42.
    • Tissue specificity

      Widely expressed. Expressed in spleen, thymus, prostate, testis, ovary, small intestine, colon and peripheral blood leukocyte. Reduced expression in breast carcinomas expressing a wild-type TP53 protein. Overexpressed in lung cancer cell lines.
    • Sequence similarities

      Belongs to the ASPP family.
      Contains 2 ANK repeats.
      Contains 1 SH3 domain.
    • Domain

      The ankyrin repeats and the SH3 domain are required for a specific interactions with TP53.
    • Cellular localization

      Cytoplasm > perinuclear region. Nucleus. Predominantly found in the perinuclear region. Some small fraction is nuclear. Sequester in the cytoplasm on overexpression of DDX42.
    • Information by UniProt
    • Database links

    • Alternative names

      • 53BP2 antibody
      • Apoptosis stimulating of p53 protein 2 antibody
      • Apoptosis stimulating protein of p53 2 antibody
      • Apoptosis-stimulating of p53 protein 2 antibody
      • ASPP2 antibody
      • ASPP2_HUMAN antibody
      • BBP antibody
      • Bcl2 binding protein antibody
      • Bcl2-binding protein antibody
      • NY REN 51 antigen antibody
      • p53 binding protein 2 antibody
      • p53-binding protein 2 antibody
      • p53BP2 antibody
      • PPP1R13A antibody
      • Renal carcinoma antigen NY-REN-51 antibody
      • Tp53bp2 antibody
      • Tumor protein p53 binding protein 2 antibody
      • Tumor suppressor p53 binding protein 2 antibody
      • Tumor suppressor p53-binding protein 2 antibody
      see all

    Images

    • All lanes : Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution (Purified)

      Lane 1 : Mouse brain lysates
      Lane 2 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 126 kDa
      Observed band size: 150 kDa
      why is the actual band size different from the predicted?



      The molecular weight observed is consistent with what has been described in PMID: 14766226 

    • Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution (Purified) + MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 126 kDa
      Observed band size: 150 kDa why is the actual band size different from the predicted?



      The molecular weight observed is consistent with what has been described in PMID: 14766226 

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human spleen tissue sections labeling 53BP2/ASPP2/BBP with purified ab181377 at 1/1000 dilution (0.15 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0). Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling 53BP2/ASPP2/BBP with ab181377 (unpurified) at a 1/100 dilution.

    • All lanes : Anti-53BP2/ASPP2/BBP antibody [EPR13837] (ab181377) at 1/10000 dilution (unpurified)

      Lane 1 : Jurkat cell lysate
      Lane 2 : Saos-2 cell lysate
      Lane 3 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 126 kDa

    • Immunohistochemical analysis of paraffin-embedded Human testis tissue labeling 53BP2/ASPP2/BBP with ab181377 (unpurified) at a 1/100 dilution.

    References

    This product has been referenced in:

    • Liu B  et al. Expression and significance of ASPP2 in squamous carcinoma of esophagus. Kaohsiung J Med Sci 34:321-329 (2018). WB, IHC-P ; Human . Read more (PubMed: 29747775) »
    See 1 Publication for this product

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