5(6)-CFDA (5(6)-Carboxyfluorescein diacetate), Amine-reactive green fluorophore (ab145278)

Overview

  • Product name

    5(6)-CFDA (5(6)-Carboxyfluorescein diacetate), Amine-reactive green fluorophore
  • Description

    Cell-permeable, amine-reactive green fluorophore
  • Biological description

    Cell-permeable, amine-reactive green fluorophore. Covalently labels intracellular proteins, peptides and nucleic acids. 5-CFDA (ab145256) and 6-CFDA (ab145298) isomer mixture. 5(6)-CFDA N-succinimidyl ester (ab145291) precursor.

  • Purity

    > 90%
  • CAS Number

    124387-19-5
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    495nm
  • Emission

    520nm
  • Chemical name

    5(6)-Carboxyfluorescein diacetate
  • Molecular weight

    460.39
  • Molecular formula

    C25H16O9
  • PubChem identifier

    44119974
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=C(C=CC(=C5)C(=O)O)C(=O)O3.CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=C(C=CC=C5C(=O)O)C(=O)O3
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Brunet A  et al. Separating the isomers--efficient synthesis of the N-hydroxysuccinimide esters of 5 and 6-carboxyfluorescein diacetate and 5 and 6-carboxyrhodamine B. Bioorg Med Chem Lett 24:3186-8 (2014). Read more (PubMed: 24856065) »
  • Gnoula C  et al. 5(6)-Carboxyfluorescein diacetate as an indicator of Caenorhabditis elegans viability for the development of an in vitro anthelmintic drug assay. Talanta 71:1886-92 (2007). Read more (PubMed: 19071538) »
  • Petroski RE & Geller HM Selective labeling of embryonic neurons cultured on astrocyte monolayers with 5(6)-carboxyfluorescein diacetate (CFDA). J Neurosci Methods 52:23-32 (1994). Read more (PubMed: 8090014) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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