5(6)-CFDA N-succinimidyl ester (CFSE), Fluorogenic esterase substrate (ab145291)

Overview

  • Product name

    5(6)-CFDA N-succinimidyl ester (CFSE), Fluorogenic esterase substrate
  • Description

    Cell-permeable, fluorogenic, non-selective esterase substrate
  • Biological description

    Cell-permeable, fluorogenic, non-selective esterase substrate. Hydrolyzes to hydrophilic fluorescent 5(6)-CFDA (ab145278) and 5(6)-FAM (ab145342) derivatives intracellularly. Partitions with high fidelity between daughter cells for up to eight generational divisions. Tracks cell proliferation.

  • Purity

    > 90%
  • CAS Number

    150347-59-4
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    495nm
  • Emission

    520nm
  • Chemical name

    5(6)-Carboxyfluorescein diacetate N-succinimidyl ester
  • Molecular weight

    557.46
  • Molecular formula

    C29H19NO11
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Eckl-Dorna J  et al. Poor association of allergen-specific antibody, T and B cell responses revealed with recombinant allergens and a CFSE dilution-based assay. Allergy : (2015). Read more (PubMed: 26043182) »
  • Lyons AB  et al. Flow cytometric analysis of cell division by dilution of CFSE and related dyes. Curr Protoc Cytom Chapter 9:Unit9.11 (2013). Read more (PubMed: 23546777) »
  • Hawkins ED  et al. Measuring lymphocyte proliferation, survival and differentiation using CFSE time-series data. Nat Protoc 2:2057-67 (2007). Read more (PubMed: 17853861) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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