5(6)-FAM (5(6)-Carboxyfluorescein), pH-senstive fluorescent label (ab145342)

Overview

  • Product name

    5(6)-FAM (5(6)-Carboxyfluorescein), pH-senstive fluorescent label
  • Description

    Amine-reactive pH-senstive fluorescent label. Covalently labels proteins and peptides.
  • Biological description

    Amine-reactive pH-sensitive fluorescent label. Covalently labels proteins and peptides. Tracks intracellular pH changes in vivo. 5-FAM (ab145293) and 6-FAM (ab145294) isomer mixture. 5(6)-CFDA (ab145278) and 5(6)-CFDA N-succinimidyl ester (ab145291) hydrolytic product.
  • Purity

    > 95%
  • CAS Number

    72088-94-9
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    492nm
  • Emission

    492nm
  • Chemical name

    5(6)-Carboxyfluorescein
  • Molecular weight

    376.32
  • Molecular formula

    C21H12O7
  • Storage instructions

    Store at Room Temperature. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Jackson VN & Halestrap AP The kinetics, substrate, and inhibitor specificity of the monocarboxylate (lactate) transporter of rat liver cells determined using the fluorescent intracellular pH indicator, 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein. J Biol Chem 271:861-8 (1996). Read more (PubMed: 8557697) »
  • Mordon S  et al. In vivo pH measurement and imaging of tumor tissue using a pH-sensitive fluorescent probe (5,6-carboxyfluorescein): instrumental and experimental studies. Photochem Photobiol 60:274-9 (1994). Read more (PubMed: 7972381) »
  • Babcock DF Examination of the intracellular ionic environment and of ionophore action by null point measurements employing the fluorescein chromophore. J Biol Chem 258:6380-9 (1983). Read more (PubMed: 6853488) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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