Key features and details
- Green fluorescent, amine-reactive label
- CAS Number: 27072-45-3
- Purity: > 90%
- Soluble in ethanol to 50 mM and in DMSO to 10 mM
- Form / State: Solid
- Source: Synthetic
Product name5(6)-FITC (Fluorescein 5(6)-isothiocyanate), Fluorescent amine-reactive label
DescriptionGreen fluorescent, amine-reactive label
Green fluorescent, amine-reactive label. Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains). Used in applications including fluorescence microscopy, flow cytometry western blotting and ELISA. 5(6)-FITC DA (ab145463) precursor. 5-FITC (ab145325) and 6-FITC (ab146082) isomer mixture.
Chemical nameFluorescein 5(6)-isothiocyanate
Storage instructionsStore at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in ethanol to 50 mM and in DMSO to 10 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab145258 has not yet been referenced specifically in any publications.