5(6)-FITC (Fluorescein 5(6)-isothiocyanate), Fluorescent amine-reactive label (ab145258)

Overview

  • Product name

    5(6)-FITC (Fluorescein 5(6)-isothiocyanate), Fluorescent amine-reactive label
  • Description

    Green fluorescent, amine-reactive label
  • Biological description

    Green fluorescent, amine-reactive label. Efficiently labels antibodies and other purified proteins at primary amines (lysine side chains). Used in applications including fluorescence microscopy, flow cytometry western blotting and ELISA. 5(6)-FITC DA (ab145463) precursor. 5-FITC (ab145325) and 6-FITC (ab146082) isomer mixture.

  • Purity

    > 90%
  • CAS Number

    27072-45-3
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    492nm
  • Emission

    518nm
  • Chemical name

    Fluorescein 5(6)-isothiocyanate
  • Molecular weight

    389.38
  • Molecular formula

    C21H11NO5S
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in ethanol to 50 mM and in DMSO to 10 mM
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Ludidi S  et al. The intestinal barrier in irritable bowel syndrome: subtype-specific effects of the systemic compartment in an in vitro model. PLoS One 10:e0123498 (2015). Read more (PubMed: 25978614) »
  • Ward DG & Cavieres JD Photoinactivation of fluorescein isothiocyanate-modified Na,K-ATPase by 2'(3')-O-(2,4,6-trinitrophenyl)8-azidoadenosine 5'-diphosphate. Abolition of E1 and E2 partial reactions by sequential block of high and low affinity nucleotide sites. J Biol Chem 273:14277-84 (1998). Read more (PubMed: 9603934) »
  • Schreiber R  et al. Effects of aniso-osmolarity and hydroperoxides on intracellular pH in isolated rat hepatocytes as assessed by (2',7')-bis(carboxyethyl)-5(6)-carboxyfluorescein and fluorescein isothiocyanate-dextran fluorescence. Biochem J 303 ( Pt 1):113-20 (1994). Read more (PubMed: 7524479) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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