Product name5,7-Dichlorokynurenic acid sodium salt, NMDA receptor glycine site antagonist
DescriptionNMDA receptor glycine site antagonist; water soluble
Biological descriptionPotent NMDA receptor glycine site antagonist. Water soluble form.
Also available in simple stock solutions (ab146691) - add 1 ml of water to get an exact, ready-to-use concentration.
Chemical name5,7-Dichloro-4-hydroxyquinoline-2-carboxylic acid sodium salt
Storage instructionsStore at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
Solubility overviewSoluble in water to 10 mM and in 1 eq. NaOH to 100 mM
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
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Our Abpromise guarantee covers the use of ab120254 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Functional Studies||Use at an assay dependent concentration.|
ab12416 staining cGMP in SKNSH cells treated with 5,7-Dichlorokynurenic acid sodium salt (ab120254), by ICC/IF. Decrease in cGMP expression correlates with increased concentration of 5,7-Dichlorokynurenic acid sodium salt, as described in literature.
The cells were incubated at 37°C for 20 minutes in media containing different concentrations of ab120254 (5,7-Dichlorokynurenic acid sodium salt) in DMSO. Some samples where then further incubated with 15 µM NMDA (ab120052) for 5 minutes and all samples were fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab12416 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with D
This product has been referenced in:
- Baron BM et al. [3H]5,7-dichlorokynurenic acid, a novel radioligand labels NMDA receptor-associated glycine binding sites. Eur J Pharmacol 206:149-54 (1991). Read more (PubMed: 1829684) »
- Baron BM et al. Activity of 5,7-dichlorokynurenic acid, a potent antagonist at the N-methyl-D-aspartate receptor-associated glycine binding site. Mol Pharmacol 38:554-61 (1990). Read more (PubMed: 2172769) »