Recombinant
RabMAb

Recombinant Anti-58K Golgi protein antibody [EPR7908] (ab131275)

Overview

  • Product name

    Anti-58K Golgi protein antibody [EPR7908]
    See all 58K Golgi protein primary antibodies
  • Description

    Rabbit monoclonal [EPR7908] to 58K Golgi protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human 58K Golgi protein aa 50-150. The exact sequence is proprietary.
    Database link: O95954

  • Positive control

    • Fetal liver lysate, Human kidney tissue and Human liver tissue.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab131275 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 58 kDa.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/1000.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
      Binds and promotes bundling of vimentin filaments originating from the Golgi.
    • Pathway

      Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
      One-carbon metabolism; tetrahydrofolate interconversion.
    • Involvement in disease

      Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
    • Sequence similarities

      In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
      In the N-terminal section; belongs to the formiminotransferase family.
    • Cellular localization

      Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
    • Information by UniProt
    • Database links

    • Alternative names

      • Formimidoyltetrahydrofolate cyclodeaminase antibody
      • Formimidoyltransferase cyclodeaminase antibody
      • Formiminotetrahydrofolate cyclodeaminase antibody
      • Formiminotransferase cyclodeaminase antibody
      • Formiminotransferase-cyclodeaminase antibody
      • FTCD antibody
      • FTCD_HUMAN antibody
      • Glutamate formiminotransferase antibody
      • Glutamate formyltransferase antibody
      • LCHC 1 antibody
      • LCHC1 antibody
      see all

    Images

    • Overlay histogram showing HepG2 cells stained with ab131275 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab131275, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.
    • Anti-58K Golgi protein antibody [EPR7908] (ab131275) at 1/1000 dilution + Human fetal liver lysate

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 58 kDa

    • Immunohistochemical analysis of paraffin-embedded Human kidney tissue labelled with ab131275 at 1/50 dilution.
    • Immunohistochemical analysis of paraffin-embedded Human liver tissue labelled with ab131275 at 1/50 dilution.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab131275 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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