Recombinant
RabMAb

Recombinant Anti-58K Golgi protein antibody [EPR7909] (ab129005)

Overview

  • Product name

    Anti-58K Golgi protein antibody [EPR7909]
    See all 58K Golgi protein primary antibodies
  • Description

    Rabbit monoclonal [EPR7909] to 58K Golgi protein
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human 58K Golgi protein aa 400-500. The exact sequence is proprietary.

  • Positive control

    • Human fetal liver lysate; Human kidney and liver tissues.
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab129005 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
      Binds and promotes bundling of vimentin filaments originating from the Golgi.
    • Pathway

      Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
      One-carbon metabolism; tetrahydrofolate interconversion.
    • Involvement in disease

      Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
    • Sequence similarities

      In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
      In the N-terminal section; belongs to the formiminotransferase family.
    • Cellular localization

      Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
    • Information by UniProt
    • Database links

    • Alternative names

      • Formimidoyltetrahydrofolate cyclodeaminase antibody
      • Formimidoyltransferase cyclodeaminase antibody
      • Formiminotetrahydrofolate cyclodeaminase antibody
      • Formiminotransferase cyclodeaminase antibody
      • Formiminotransferase-cyclodeaminase antibody
      • FTCD antibody
      • FTCD_HUMAN antibody
      • Glutamate formiminotransferase antibody
      • Glutamate formyltransferase antibody
      • LCHC 1 antibody
      • LCHC1 antibody
      see all

    Images

    • Anti-58K Golgi protein antibody [EPR7909] (ab129005) at 1/1000 dilution + Human fetal liver lyaste at 10 µg

      Secondary
      Goat anti-Rabbit HRP at 1/2000 dilution

      Predicted band size: 58 kDa
      Observed band size: 58 kDa

    • ab129005, at 1/250 dilution, staining 58K Golgi protein in paraffin-embedded Human kidney tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • ab129005, at 1/250 dilution, staining 58K Golgi protein in paraffin-embedded Human liver tissue by Immunohistochemistry.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab129005 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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