Key features and details
- Goat polyclonal to 58K Golgi protein - Golgi Marker
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-58K Golgi protein antibody - Golgi Marker
See all 58K Golgi protein primary antibodies
DescriptionGoat polyclonal to 58K Golgi protein - Golgi Marker
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Pig
- Human liver lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab19072 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.03 - 0.2 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 59 kDa).
A 1 hour primary incubation is recommended for this product.
|IHC-P||Use a concentration of 3 - 5 µg/ml. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. PubMed: 18315599|
FunctionFolate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Binds and promotes bundling of vimentin filaments originating from the Golgi.
PathwayAmino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion.
Involvement in diseaseDefects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities.
Sequence similaritiesIn the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
In the N-terminal section; belongs to the formiminotransferase family.
Cellular localizationCytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole.
- Information by UniProt
- Formimidoyltetrahydrofolate cyclodeaminase antibody
- Formimidoyltransferase cyclodeaminase antibody
- Formiminotetrahydrofolate cyclodeaminase antibody
ab19072 at 3.8µg/ml staining 58K Golgi protein in human liver tissue section by Immunohistochemistry (Formalin-PFA fixed paraffin-embedded tissue sections). Steamed antigen retrieval in citrate buffer pH 6 was performed. AP staining procedure was used for detection.
Anti-58K Golgi protein antibody - Golgi Marker (ab19072) at 0.05 µg/ml + Human liver lysate at 35 µg
Predicted band size: 59 kDa
ab19072 has been referenced in 6 publications.
- Perland E et al. The Novel Membrane-Bound Proteins MFSD1 and MFSD3 are Putative SLC Transporters Affected by Altered Nutrient Intake. J Mol Neurosci 61:199-214 (2017). PubMed: 27981419
- Li H et al. Synaptic vesicles contain small ribonucleic acids (sRNAs) including transfer RNA fragments (trfRNA) and microRNAs (miRNA). Sci Rep 5:14918 (2015). WB . PubMed: 26446566
- Tinker JK et al. Purification and characterization of Yersinia enterocolitica and Yersinia pestis LcrV-cholera toxin A(2)/B chimeras. Protein Expr Purif 74:16-23 (2010). PubMed: 20438844
- Schrage YM et al. Aberrant heparan sulfate proteoglycan localization, despite normal exostosin, in central chondrosarcoma. Am J Pathol 174:979-88 (2009). IHC-P ; Human . PubMed: 19179614
- Willems SM et al. Myxoid tumours of soft tissue: the so-called myxoid extracellular matrix is heterogeneous in composition. Histopathology 52:465-74 (2008). IHC-P ; Human . PubMed: 18315599
- Zahn C et al. ADP-ribosylation Factor-like GTPase ARFRP1 Is Required for Trans-Golgi to Plasma Membrane Trafficking of E-cadherin. J Biol Chem 283:27179-27188 (2008). IHC ; Mouse . PubMed: 18662990