Anti-58K Golgi protein antibody - N-terminal (Biotin) (ab214784)
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Overview
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Product name
Anti-58K Golgi protein antibody - N-terminal (Biotin)
See all 58K Golgi protein primary antibodies -
Description
Goat polyclonal to 58K Golgi protein - N-terminal (Biotin) -
Host species
Goat -
Conjugation
Biotin -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Pig, Dictyostelium discoideum
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Immunogen
Synthetic peptide corresponding to Human 58K Golgi protein aa 2-17 (N terminal). (NP_006648.1).
Sequence:SQLVECVPNFSEGKNQ
Database link: O95954 -
Positive control
- Human liver lysate.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab214784 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab214784 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 59 kDa). |
Target
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Function
Folate-dependent enzyme, that displays both transferase and deaminase activity. Serves to channel one-carbon units from formiminoglutamate to the folate pool.
Binds and promotes bundling of vimentin filaments originating from the Golgi. -
Pathway
Amino-acid degradation; L-histidine degradation into L-glutamate; L-glutamate from N-formimidoyl-L-glutamate (transferase route): step 1/1.
One-carbon metabolism; tetrahydrofolate interconversion. -
Involvement in disease
Defects in FTCD are the cause of glutamate formiminotransferase deficiency (FIGLU-URIA) [MIM:229100]; also known as formiminoglutamicaciduria (FIGLU-uria). It is an autosomal recessive disorder. Features of a severe phenotype, include elevated levels of formiminoglutamate (FIGLU) in the urine in response to histidine administration, megaloblastic anemia, and mental retardation. Features of a mild phenotype include high urinary excretion of FIGLU in the absence of histidine administration, mild developmental delay, and no hematological abnormalities. -
Sequence similarities
In the C-terminal section; belongs to the cyclodeaminase/cyclohydrolase family.
In the N-terminal section; belongs to the formiminotransferase family. -
Cellular localization
Cytoplasm > cytoskeleton > centrosome > centriole. Golgi apparatus. More abundantly located around the mother centriole. - Information by UniProt
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Database links
- Entrez Gene: 8626393 Dictyostelium discoideum
- Entrez Gene: 10841 Human
- Entrez Gene: 14317 Mouse
- Entrez Gene: 397517 Pig
- Entrez Gene: 89833 Rat
- Omim: 606806 Human
- SwissProt: Q54JL3 Dictyostelium discoideum
- SwissProt: O95954 Human
see all -
Alternative names
- Formimidoyltetrahydrofolate cyclodeaminase antibody
- Formimidoyltransferase cyclodeaminase antibody
- Formiminotetrahydrofolate cyclodeaminase antibody
see all
Images
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Western blot - Anti-58K Golgi protein antibody - N-terminal (Biotin) (ab214784)Anti-58K Golgi protein antibody - N-terminal (Biotin) (ab214784) at 1 µg/ml + Human liver lysate at 35 µg
Secondary
Streptavidin-HRP
Developed using the ECL technique.
Predicted band size: 59 kDa
Protocols
Datasheets and documents
References
ab214784 has not yet been referenced specifically in any publications.
