Anti-5HT1B Receptor antibody (ab52025)
Key features and details
- Goat polyclonal to 5HT1B Receptor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-5HT1B Receptor antibody
See all 5HT1B Receptor primary antibodies -
Description
Goat polyclonal to 5HT1B Receptor -
Host species
Goat -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Chimpanzee, Syrian hamster -
Immunogen
Synthetic peptide:
C-SNEDFKQAFHK
, corresponding to C terminal amino acids 372-382 of Human 5HT1B Receptor -
Positive control
- Human Brain lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab52025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Post-translational
modificationsPhosphorylated. Desensitization of the receptor may be mediated by its phosphorylation.
Palmitoylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 100144391 Cat
- Entrez Gene: 462833 Chimpanzee
- Entrez Gene: 403741 Dog
- Entrez Gene: 100135543 Guinea pig
- Entrez Gene: 100009680 Horse
- Entrez Gene: 3351 Human
- Entrez Gene: 15551 Mouse
- Entrez Gene: 100009594 Rabbit
see all -
Alternative names
- 5 HT1DB antibody
- 5 hydroxytryptamine receptor 1B antibody
- 5-HT-1B antibody
see all
Images
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Anti-5HT1B Receptor antibody (ab52025) at 2 µg/ml + Human Brain lysate (35µg protein in RIPA buffer).
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
Datasheets and documents
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Datasheet download
References (0)
ab52025 has not yet been referenced specifically in any publications.