Anti-5-HT2C Receptor antibody (ab32887)
Key features and details
- Goat polyclonal to 5-HT2C Receptor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-5-HT2C Receptor antibody
See all 5-HT2C Receptor primary antibodies -
Description
Goat polyclonal to 5-HT2C Receptor -
Host species
Goat -
Specificity
Ab32887 recognises 5HT2C Receptor. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Dog, Chimpanzee -
Immunogen
Synthetic peptide:
C-QVENLELPVN
, corresponding to amino acids 437-446 of Human 5HT2C Receptor. -
Positive control
- Lysates of EBV immortalised Lymphoblastoids, T cell, MOLT4 and monocytic cell line U937.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris buffered saline, 0.5% BSA -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Ab32887 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab32887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa).
1 hour primary incubation is recommended for this product. |
Notes |
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WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 52 kDa). 1 hour primary incubation is recommended for this product. |
Target
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Function
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Domain
The PDZ domain-binding motif is involved in the interaction with MPDZ. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 473740 Chimpanzee
- Entrez Gene: 450240 Dog
- Entrez Gene: 3358 Human
- Entrez Gene: 15560 Mouse
- Entrez Gene: 25187 Rat
- Omim: 312861 Human
- SwissProt: Q5IS66 Chimpanzee
- SwissProt: Q60F97 Dog
see all -
Alternative names
- 5 Hydroxytryptamine 2C receptor antibody
- 5-HT-1C antibody
- 5-ht-1c receptor antibody
see all
Images
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Anti-5-HT2C Receptor antibody (ab32887) at 1 µg/ml + EBV immortalised Lymphoblastoid lysate in RIPA buffer at 35 µg
Developed using the ECL technique.
Predicted band size: 52 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 1 minute
Protocols
Datasheets and documents
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Datasheet download
References (3)
ab32887 has been referenced in 3 publications.
- Price AE et al. Endogenous Serotonin 5-HT2A and 5-HT2C Receptors Associate in the Medial Prefrontal Cortex. ACS Chem Neurosci 10:3241-3248 (2019). PubMed: 30645940
- Delicata F et al. Preferential modulation of the lateral habenula activity by serotonin-2A rather than -2C receptors: Electrophysiological and neuroanatomical evidence. CNS Neurosci Ther 24:721-733 (2018). PubMed: 29479825
- Ji G et al. 5-HT2CReceptor Knockdown in the Amygdala Inhibits Neuropathic-Pain-Related Plasticity and Behaviors. J Neurosci 37:1378-1393 (2017). PubMed: 28011743