Product nameAnti-5HT6 Receptor antibody
See all 5HT6 Receptor primary antibodies
DescriptionRabbit polyclonal to 5HT6 Receptor
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Synthetic peptide corresponding to Mouse 5HT6 Receptor aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available as
- This antibody gave a positive signal in the following tissue lysates: Mouse dorsal root ganglion; Mouse brain P0; Mouse brain P7. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse Brain.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab103016 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).|
FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. It has a high affinity for tricyclic psychotropic drugs.
Tissue specificityExpressed in several human brain regions, most prominently in the caudate nucleus.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- 5 HT 6 antibody
- 5 HT6 antibody
- 5 HT6R antibody
IHC image of 5HT6 Receptor staining in Mouse Brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103016, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
All lanes : Anti-5HT6 Receptor antibody (ab103016) at 1 µg/ml
Lane 1 : Dorsal Root Ganglia (Mouse) Tissue Lysate
Lane 2 : P0 Mouse Brain Mouse Tissue Lysate
Lane 3 : P7 Mouse Brain Tissue Lysate
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?
Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
5HT6 Receptor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.
This product has been referenced in:
- Teng LL et al. Serotonin receptor HTR6-mediated mTORC1 signaling regulates dietary restriction-induced memory enhancement. PLoS Biol 17:e2007097 (2019). Read more (PubMed: 30883547) »
- Guo J et al. Primary Cilia Signaling Shapes the Development of Interneuronal Connectivity. Dev Cell 42:286-300.e4 (2017). Read more (PubMed: 28787594) »