Overview

  • Product name

    Anti-5HT6 Receptor antibody
    See all 5HT6 Receptor primary antibodies
  • Description

    Rabbit polyclonal to 5HT6 Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, WBmore details
  • Species reactivity

    Reacts with: Mouse
  • Immunogen

    Synthetic peptide corresponding to Mouse 5HT6 Receptor aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab117404)

  • Positive control

    • This antibody gave a positive signal in the following tissue lysates: Mouse dorsal root ganglion; Mouse brain P0; Mouse brain P7. This antibody gave a positive result in IHC in the following FFPE tissue: Mouse Brain.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab103016 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 0.5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 47 kDa).

Target

  • Function

    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. It has a high affinity for tricyclic psychotropic drugs.
  • Tissue specificity

    Expressed in several human brain regions, most prominently in the caudate nucleus.
  • Sequence similarities

    Belongs to the G-protein coupled receptor 1 family.
  • Cellular localization

    Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5 HT 6 antibody
    • 5 HT6 antibody
    • 5 HT6R antibody
    • 5 hydroxytryptamine (serotonin) receptor 6 G protein coupled antibody
    • 5 hydroxytryptamine 6 receptor antibody
    • 5 hydroxytryptamine receptor 6 antibody
    • 5-HT-6 antibody
    • 5-HT6 antibody
    • 5-hydroxytryptamine receptor 6 antibody
    • 5HT 6 antibody
    • 5HT6R_HUMAN antibody
    • HTR 6 antibody
    • Htr6 antibody
    • Serotonin receptor 6 antibody
    see all

Images

  • IHC image of 5HT6 Receptor staining in Mouse Brain formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab103016, 0.5 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • All lanes : Anti-5HT6 Receptor antibody (ab103016) at 1 µg/ml

    Lane 1 : Dorsal Root Ganglia (Mouse) Tissue Lysate
    Lane 2 : P0 Mouse Brain Mouse Tissue Lysate
    Lane 3 : P7 Mouse Brain Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 55 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 41 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 30 seconds


    5HT6 Receptor contains a potential glycosylation site (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

This product has been referenced in:

  • Teng LL  et al. Serotonin receptor HTR6-mediated mTORC1 signaling regulates dietary restriction-induced memory enhancement. PLoS Biol 17:e2007097 (2019). Read more (PubMed: 30883547) »
  • Guo J  et al. Primary Cilia Signaling Shapes the Development of Interneuronal Connectivity. Dev Cell 42:286-300.e4 (2017). Read more (PubMed: 28787594) »
See all 4 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Answer

Thank you very much for your interest in ab103016.

To our knowledge, ab103016 has not been tested in ICC/IF. We do not have any small aliquots for testing. Therefore, I can offer a discount off a future purchase if you buy ab103016 now, test it inICC/IF and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: 1 free primary antibody.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab103016 in ICC/IF. I will then send a discount code. This code must be issued before purchasing ab103016 so please wait for my reply before ordering.

2. Purchase ab103016 either by phone, fax, or online (www.abcam.com).

3. Test it in ICC/IF.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for anyprimary antibodyordered and the discount code is valid for 4 months after issue. Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab103016 turns out to be unsuitable for ICC/IF, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: https://www.abcam.com/collaborationdiscount.

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Answer

Merci pour votre réponse.
J'ai comme convenu mis en place l'envoi d'une unité d'anti-5HT6 ab101912. Le numéro de commande de remplacement gratuit de ce produit : *****. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.
N'hésitez pas à nous contacter lors d'une prochaine occasion.

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Answer

Merci de nous avoir contactés et désolé pour le délai de notre réponse.
J'ai contacté mes collègues du laboratoire, ils n'ont malheureusement pas testé cet anticorps avec un contrôle négatif. Les tests de caractérisation montrent une bande à ˜55 kDa avec des lysats de cerveau de souris.
Afin de comparer et confirmer ou non les résultats obtenus avec le ab103016 je peux, si vous le désirez, vous envoyer une unité gratuite de ab101912, un autre anti-récepteur 5HT6 testé en Western Blot et chez la souris: www.abcam.com/ab101912 .
Merci de me confirmer que vous acceptez cette résolution. Alternativement je peux également éditer un avoir contre la commande originale.

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