Anti-5HT1A Receptor antibody (ab227165)
Key features and details
- Rabbit polyclonal to 5HT1A Receptor
- Suitable for: ICC/IF, WB
- Reacts with: Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-5HT1A Receptor antibody
See all 5HT1A Receptor primary antibodies -
Description
Rabbit polyclonal to 5HT1A Receptor -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Rat, Human
Predicted to work with: Chimpanzee, Rhesus monkey -
Immunogen
Synthetic peptide within Human 5HT1A Receptor (internal sequence). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P08908 -
Positive control
- WB: MOLT-4 and Raji whole cell lysates; D-hippocampus C9 lysate. ICC/IF: DIV14 rat E18 primary cortical neurons.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 59.99% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab227165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 46 kDa.
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Notes |
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ICC/IF
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 46 kDa. |
Target
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Function
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3350 Human
- Entrez Gene: 24473 Rat
- Omim: 109760 Human
- SwissProt: P08908 Human
- SwissProt: P19327 Rat
- Unigene: 247940 Human
- Unigene: 44486 Rat
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Alternative names
- 5 HT 1A antibody
- 5 HT receptor 1A antibody
- 5 HT1A antibody
see all
Images
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All lanes : Anti-5HT1A Receptor antibody (ab227165) at 1/1000 dilution
Lane 1 : MOLT-4 (human lymphoblastic leukemia cell line) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 46 kDa10% SDS-PAGE gel.
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4% paraformaldehyde-fixed DIV14 rat E18 primary cortical neurons stained for 5HT1A (green) using ab227165 at 1/500 dilution in ICC/IF. Counterstain: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody at 1/500 dilution (red). Nuclear Counterstain: Fluoroshield with DAPI (blue).
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Anti-5HT1A Receptor antibody (ab227165) at 1/1000 dilution + D-hippocampus C9 lysate at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 46 kDa10% SDS-PAGE gel.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab227165 has been referenced in 2 publications.
- Zhang B et al. Environmentally-relevant concentrations of the antipsychotic drugs sulpiride and clozapine induce abnormal dopamine and serotonin signaling in zebrafish brain. Sci Rep 12:17973 (2022). PubMed: 36289270
- Yahfoufi N et al. Adolescent use of potential novel probiotic Rouxiella badensis subsp. acadiensis (Canan SV-53) mitigates pubertal LPS-Induced behavioral changes in adulthood in a sex-specific manner by modulating 5HT1A receptors expression in specific brain areas. Compr Psychoneuroendocrinol 7:100063 (2021). PubMed: 35757063