• Product name
    Anti-5HT1A Receptor antibody
    See all 5HT1A Receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT1A Receptor
  • Host species
  • Tested applications
    Suitable for: IHC-FoFr, WB, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
    Predicted to work with: Horse, Chicken, Dog, Chimpanzee, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Rat 5HT1A Receptor aa 100-200 conjugated to keyhole limpet haemocyanin.
    Database link: P19327
    (Peptide available as ab98259)

  • Positive control
    • This antibody gave a positive signal in both Mouse and Rat hippocampus tissue lysate as well as the following whole cell lysates: Caco2; HepG2; Jurkat. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal liver.



Our Abpromise guarantee covers the use of ab85615 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr Use at an assay dependent concentration. PubMed: 26076134
WB Use a concentration of 1 µg/ml. Detects a band of approximately 62 kDa (predicted molecular weight: 46 kDa).
IHC-P Use a concentration of 5 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.


  • Function
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family. 5-hydroxytryptamine receptor subfamily. HTR1A sub-subfamily.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 HT 1A antibody
    • 5 HT receptor 1A antibody
    • 5 HT1A antibody
    • 5 HT1A receptor antibody
    • 5 hydroxytryptamine (serotonin) receptor 1A antibody
    • 5 hydroxytryptamine (serotonin) receptor 1A G protein coupled antibody
    • 5 hydroxytryptamine receptor 1A antibody
    • 5-HT-1A antibody
    • 5-HT1A antibody
    • 5-hydroxytryptamine receptor 1A antibody
    • 5HT 1A antibody
    • 5HT 1A receptor antibody
    • 5HT receptor 1A antibody
    • 5HT1a antibody
    • 5HT1A_HUMAN antibody
    • ADRB2RL1 antibody
    • ADRBRL1 antibody
    • G 21 antibody
    • G protein coupled receptor antibody
    • G-21 antibody
    • Gpcr 18 antibody
    • Gpcr18 antibody
    • Guanine nucleotide binding regulatory protein-coupled receptor antibody
    • Htr1a antibody
    • PFMCD antibody
    • Serotonin 1A receptor antibody
    • Serotonin 5HT1A receptor antibody
    • Serotonin receptor 1A antibody
    see all


  • IHC image of 5HT1A Receptor staining in Human normal liver formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab85615, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • ICC/IF image of ab85615 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85615, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  • Immunohistochemistry (PFA perfusion fixed frozen sections) analysis of rat hippocampus labelling 5HT1A with ab85615 (red). For immunofluorescence analysis, the rats were anaesthetized using chloral hydrate and then were perfused with 150 mL of saline solution and 150–200 mL of 4% paraformaldehyde in 0.1 M phosphate buffer (PB). Then, the brains of the rats were isolated and fixed in 4% paraformaldehyde for 2–3 h. The rat brain sections were cut on a freezing microtome at a thickness of 16 μm and were washed with phosphate-buffered saline (PBS) and pre-incubated in 5% horse serum. Subsequently, the sections were incubated with Anti-5HT1A Receptor antibody (ab85615) for 24 h at 4°C, washed with PBS and incubated with the secondary antibody and neuro tracer for 2 h at room temperature while protected from light. The sections were examined on a Nikon C2 plus confocal laser-scanning microscope.

  • All lanes : Anti-5HT1A Receptor antibody (ab85615) at 1 µg/ml

    Lane 1 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 2 : Rat Hippocampus Tissue Lysate
    Lane 3 : Mouse Hippocampus Tissue Lysate
    Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 5 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 46 kDa
    Observed band size: 62 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 37 kDa, 48 kDa. We are unsure as to the identity of these extra bands.

    Exposure time: 30 seconds

    The expression profile observed is consistent with what has been described in the literature (PMID: 16772521).


This product has been referenced in:
  • Cui WQ  et al. Spinal Serotonin 1A Receptor Contributes to the Analgesia of Acupoint Catgut Embedding by Inhibiting Phosphorylation of the N-Methyl-d-Aspartate Receptor GluN1 Subunit in Complete Freund's Adjuvant-Induced Inflammatory Pain in Rats. J Pain 20:16.e1-16.e16 (2019). Read more (PubMed: 30102991) »
  • Jo K  et al. Polygonatum sibiricum rhizome promotes sleep by regulating non-rapid eye movement and GABAergic/serotonergic receptors in rodent models. Biomed Pharmacother 105:167-175 (2018). WB ; Rat . Read more (PubMed: 29857295) »
See all 8 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Frozen sections)
Horse Tissue sections (mucosa of the ileum)
Yes - Triton
mucosa of the ileum
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 20°C

Chiocchetti Roberto

Verified customer

Submitted May 23 2019

Immunohistochemistry free floating
Mouse Tissue sections (brain)

Mr. Jacky Liang

Verified customer

Submitted Jun 28 2018


I am waiting for replies from some of the laboratories that produced these antibodies.

Of the antibodies for which we know the immunogen sequence (others are considered proprietary by the laboratories that produce the antibodies), the only two that have immunogen sequences with greater than 50% identity with the honey bee sequence you sent are:

ab85615, anti 5HT1A receptor, 77% (10/13 identical amino acids) and

ab60359, anti- 5HT4 receptor, 52% (22/42 identical amino acids).

Read More
Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat Tissue sections (Brain)
Antigen retrieval step

Dr. Sophie Pezet

Verified customer

Submitted Jul 06 2012

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