Overview

  • Product name
    Anti-5HT1B Receptor antibody
    See all 5HT1B Receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT1B Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, IHC-Frmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Rat 5HT1B Receptor. 8-26(CAPPPPATSQTGVPLANLS) and 263-278(VTSINSRVPEVPSESG)
    Database link: P28564

  • Positive control
    • Tested with human brain lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab13896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40-41 kDa (predicted molecular weight: 47 kDa).
IHC-P Use a concentration of 4 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration. PubMed: 24642693

Target

  • Function
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Post-translational
    modifications
    Phosphorylated. Desensitization of the receptor may be mediated by its phosphorylation.
    Palmitoylated.
  • Cellular localization
    Cell membrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 HT1DB antibody
    • 5 hydroxytryptamine receptor 1B antibody
    • 5-HT-1B antibody
    • 5-HT-1D-beta antibody
    • 5-HT1B antibody
    • 5-hydroxytryptamine receptor 1B antibody
    • 5HT1B_HUMAN antibody
    • Htr1b antibody
    • HTR1D2 antibody
    • HTR1DB antibody
    • S12 antibody
    • Serotonin 1D beta receptor antibody
    • Serotonin receptor 1B antibody
    see all

Images

  • ab17976 staining 5HT1B in Human colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with 10% NBFand blocked with antibody block for 10 minutes; antigen retrieval was by heat mediation in CC1 Mild. Samples were incubated with primary antibody (1/100 in antibody diluent) for 1 hour at 37°C. An undiluted Biotin-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

  • Western blot analysis of 5HT1B Receptor in human brain lysate with ab13896. A protein band of approximate molecular weight of 40-41kDa was detected. Western blot analysis of 5HT1B Receptor in human brain lysate with ab13896. A protein band of approximate molecular weight of 40-41kDa was detected.
  • ab2607 (4µg/ml) staining 5HT1B in human testis using an automated system (DAKO Autostainer Plus). Using this protocol there is strong staining of membrane compartment of the mature seminiferous tubules and possible interstitial cells.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

References

This product has been referenced in:
  • Han X  et al. Electroacupuncture restores hippocampal synaptic plasticity via modulation of 5-HT receptors in a rat model of depression. Brain Res Bull 139:256-262 (2018). WB . Read more (PubMed: 29524471) »
  • Li Y  et al. Pericytes impair capillary blood flow and motor function after chronic spinal cord injury. Nat Med 23:733-741 (2017). IHC ; Rat . Read more (PubMed: 28459438) »
See all 15 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain, prefrontal cortex and caudate putamen)
Permeabilization
Yes - Triton-X 100
Specification
Brain, prefrontal cortex and caudate putamen
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
At excision, the cerebellum was removed, the brain was divided in hemispheres and frozon in isopentane cooled on dry ice. Before IHC, sections were postfixated for 5 min in 96 % EtOH

Abcam user community

Verified customer

Submitted Oct 06 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Antibody Block as blocking agent for 10 minute(s) · Concentration: 100%
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: CC1 Mild
Sample
Human Tissue sections (Colon)
Specification
Colon
Permeabilization
No
Fixative
10% NBF

Abcam user community

Verified customer

Submitted Dec 16 2013

Answer

Thank you for your response.

The information provided in the two e-mails is very limited. In the Lab, this antibody has been tested and characterized for Western blot application on human brain tissue lysate and not on SHySy5 cells. You may need to test different dilutions 1/200, 1/100 and/or load more protein.

Unfortunately, It is not clear how much protein was loaded onto the gel. Have you checked the detection system? Is the secondary antibody compatible with the primary antibody?

If you wish to proceed with this enquiry, it would be appreciated to complete the attached Questionnaire.

I am particularly interested in the following information:

- Sample preparation and lysis buffer;

- Total cell lysate or membrane fractions used,

- Loaded amount of protein;

- Blocking conditions

- Host species and specificity of the secondary antibody used,

- Positive control i.e human total brain lysate,

- Order Number and Your PON (order confirmation),

- Date of arrival

I hope this will be useful for you. Should you still have any problem with this antibody after following these suggestions, then please do not hesitate to contact our Technical Department again.

Read More

Answer

I am sorry to hear that you have been experiencing problems using this product in the application that you wish.

In order to assess the quality of our products I would ask that you complete a brief questionnaire relating to the application used. Often it is possible to make suggestions that may help resolve problems experienced using a particular product.

As our Abpromise indicates, in the event that a product is not functioning in the applications/species cited on the product data sheet (and the problem has been reported within 6 months of purchase) we will happily offer a credit note/refund to the value of the product purchased.

All our customer feedback, including complaints are monitored weekly by our in house technical support team. If a product is at fault the technical support team will consider removing the product from our catalogue in order to avoid future customer inconvenience.

Could you provide some further details of the protocol used and complete the following form (attached as a word document). It would be much appreciated if you could attach an image to the response.

Thank you for your understanding and co-operation in this matter. I look forward to hearing from you soon and resolving this issue as soon as possible.

Read More

Answer

Thank you for contacting us.
The immunogens are not commercially available at this time.
Please let us know if you have any other questions.

Read More

Answer

Thanks for your reply.

It is good to know that you have used the Trizol extraction method successfully with other samples. The reason I suggested the negative control sample was because it would, if there is an issue with the antibody specificity here, it would be confirmed with the presence of many non-specific bands in a sample that does not express 5HT1B. However if a blot against this negative control sample is blank or has one of the intense bands is missing as compared to the hamster brain samples, that could confirm there is specific detection of 5HT1B and perhaps further optimization may then be worthwhile.

What do you think?

Thanks for your troubleshooting efforts!

Read More

Answer

Thanks again for submitting your protocol details as well as the western blotting image.

I just have a couple additional questions:

1) Have you run a negative control sample that you expect would not contain 5HT1B?

2) Have you used these same brain extracts in western blots with any other antibodies?

Thanks for the additional information!

Read More

Answer

Thank you for your enquiry. I am sorry to hear this vial of ab13896 is giving you trouble. I am currently reviewing the information you have generously provided and will follow up with you as soon as possible.

Thanks for your patience!

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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