Key features and details
- Goat polyclonal to 5HT1B Receptor
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-5HT1B Receptor antibody
See all 5HT1B Receptor primary antibodies
DescriptionGoat polyclonal to 5HT1B Receptor
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cat, Dog, Chimpanzee, Syrian hamster
- Human Brain lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 0.5% Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab52025 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 48 kDa (predicted molecular weight: 44 kDa).
1 hour primary incubation is recommended for this product.
FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
modificationsPhosphorylated. Desensitization of the receptor may be mediated by its phosphorylation.
Cellular localizationCell membrane.
- Information by UniProt
- 5 HT1DB antibody
- 5 hydroxytryptamine receptor 1B antibody
- 5-HT-1B antibody
Anti-5HT1B Receptor antibody (ab52025) at 2 µg/ml + Human Brain lysate (35µg protein in RIPA buffer).
Predicted band size: 44 kDa
Observed band size: 48 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
ab52025 has not yet been referenced specifically in any publications.