Overview

  • Product name

    Anti-5HT1D Receptor antibody
  • Description

    Rabbit polyclonal to 5HT1D Receptor
  • Host species

    Rabbit
  • Specificity

    BLAST analysis of the peptide immunogen showed no homology with other Human proteins.
  • Tested applications

    Suitable for: IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Monkey, Gorilla, Common marmoset, Bat
  • Immunogen

    Synthetic peptide, corresponding to a 16 amino acid region from the third cytoplasmic domain of Human 5HT1D Receptor (NP_000855.1).

  • Positive control

    • Human spinal cord, dorsal root ganglion tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab140486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 - 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • Immunohistochemical analysis of formalin fixed, paraffin embedded Human spinal cord, dorsal root ganglion tissue labelling 5HT1D Receptor with ab140486 at 17 µg/ml.

References

This product has been referenced in:

  • Yoshihara M  et al. Discovery of molecular markers to discriminate corneal endothelial cells in the human body. PLoS One 10:e0117581 (2015). Read more (PubMed: 25807145) »
See 1 Publication for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Corneal endothelial cells)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate pH 6
Permeabilization
Yes - Triton X-100
Specification
Corneal endothelial cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jul 06 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Rabbit Cell (Corneal endothelial cells)
Permeabilization
Yes - Triton X-100
Specification
Corneal endothelial cells
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Jun 27 2016

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