Product nameAnti-5HT1F Receptor antibody
See all 5HT1F Receptor primary antibodies
DescriptionRabbit polyclonal to 5HT1F Receptor
Specificityab28695 recognises hydroxytryptamine 1F receptor (HTR1F).
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Pig, Chimpanzee
- Fetal liver lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab28695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
Relevance5HT1F is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. 5HT1F is a member of the GPCR family (subfamily: Serotonin). Expression of 5HT1F has been reported in various brain structures, including cortex, hippocampus, trigeminal ganglia, and cerebral blood vessels. Expression in peripheral tissues is limited to uterus, mesentery, and artery. No expression has been detected in heart, kidney, liver, pancreas, or spleen.
Cellular localizationCell membrane; Multi pass membrane protein.
- 5 HT 1F antibody
- 5 HT1F antibody
- 5 hydroxytryptamine (serotonin) receptor 1F antibody
Anti-5HT1F Receptor antibody (ab28695) at 2.5 µg/ml + Fetal liver lysate
HRP conjugated anti-Rabbit IgG
Predicted band size: 44 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Western Blot using ab28695 and a gel concentration of 12%
ab28695 has not yet been referenced specifically in any publications.