• Product name

    Anti-5HT1F Receptor antibody
    See all 5HT1F Receptor primary antibodies
  • Description

    Rabbit polyclonal to 5HT1F Receptor
  • Host species

  • Specificity

    ab28695 recognises hydroxytryptamine 1F receptor (HTR1F).
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Pig, Chimpanzee
  • Immunogen

    A region within a synthetic peptide: MDFLNSSDQN LTSEELLNRM PSKILVSLTL SGLALMTTTI NSLVIAAIIV, corresponding to N terminal amino acids 1-50 of Human 5HT1F Receptor

  • Positive control

    • Fetal liver lysate



Our Abpromise guarantee covers the use of ab28695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2.5 µg/ml. Detects a band of approximately 42 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA 1/62500.


  • Relevance

    5HT1F is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that inhibit adenylate cyclase activity. 5HT1F is a member of the GPCR family (subfamily: Serotonin). Expression of 5HT1F has been reported in various brain structures, including cortex, hippocampus, trigeminal ganglia, and cerebral blood vessels. Expression in peripheral tissues is limited to uterus, mesentery, and artery. No expression has been detected in heart, kidney, liver, pancreas, or spleen.
  • Cellular localization

    Cell membrane; Multi pass membrane protein.
  • Database links

  • Alternative names

    • 5 HT 1F antibody
    • 5 HT1F antibody
    • 5 hydroxytryptamine (serotonin) receptor 1F antibody
    • 5 hydroxytryptamine 1F receptor antibody
    • 5 hydroxytryptamine receptor 1E beta antibody
    • 5 hydroxytryptamine receptor 1F antibody
    • 5 hydroxytryptamine serotonin receptor 1E beta antibody
    • 5 hydroxytryptamine serotonin receptor 1F antibody
    • 5HT 1F antibody
    • 5HT1F antibody
    • HGNC5292 antibody
    • HTR 1F antibody
    • HTR1EB antibody
    • HTR1EL antibody
    • HTR1F antibody
    • MR 77 antibody
    • MR77 antibody
    • Serotonin receptor 1E beta antibody
    • Serotonin receptor gene antibody
    see all


  • Anti-5HT1F Receptor antibody (ab28695) at 2.5 µg/ml + Fetal liver lysate

    HRP conjugated anti-Rabbit IgG

    Predicted band size: 44 kDa
    Observed band size: 42 kDa
    why is the actual band size different from the predicted?

    Western Blot using ab28695 and a gel concentration of 12%


ab28695 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


I am pleased to confirm that the immunogen sequence used to generate ab28695 is 93% homologous to mouse HTR1F. The antibody has therefore good chances of detecting mouse protein, however we cannot guarantee this as it has not been tested. It is very unlikely that ab26092 would cross react with the TP5H1 form according to the BLAST results of immunogen sequence, however the antibody has not specifically been tested against this form of the protein to see if any cross reaction would occur. I hope this information will be useful, please do not hesitate to contact us if you need further assistance.

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