Overview

  • Product name
    Anti-5HT2A Receptor antibody
    See all 5HT2A Receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT2A Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC-FoFr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide:

    GDGPRLYHNDFNSRDANTSE

    , corresponding to N terminal amino acids 22-41 of Rat 5HT2A Receptor

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Rat cortex, amygdala and hippocampus tissue; mouse frontal cortical neuron cultures.
  • General notes
    Upon delivery dilute with phosphate buffer or Tris buffer at dilutions no higher than 1/10, aliquot and freeze at -15° C or lower. Antibody can be stored for up to six months if handled as described above.

Properties

Applications

Our Abpromise guarantee covers the use of ab66049 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-FoFr 1/300 - 1/500. Biotin/Streptavidin-HRP Technique.
WB 1/100. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).

Target

  • Function
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Domain
    The PDZ domain-binding motif is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4.
  • Cellular localization
    Cell membrane. Localizes to the post-synaptic thickening of axo-dendritic synapses.
  • Information by UniProt
  • Database links
    see all
  • Alternative names
    • 5 HT 2 antibody
    • 5 HT 2A antibody
    • 5 HT2 receptor antibody
    • 5 HT2A antibody
    • 5 hydroxytryptamine receptor 2A antibody
    • 5-HT-2 antibody
    • 5-HT-2A antibody
    • 5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled antibody
    • 5-hydroxytryptamine 2A receptor antibody
    • 5-hydroxytryptamine receptor 2A antibody
    • 5HT2A_HUMAN antibody
    • HTR 2 antibody
    • HTR 2A antibody
    • HTR2 antibody
    • HTR2, formerly antibody
    • HTR2A antibody
    • serotonin 5-HT-2 receptor, formerly antibody
    • serotonin 5-HT-2A receptor antibody
    • Serotonin receptor 2A antibody
    see all

Images

  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-5HT2A Receptor antibody (ab66049)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-5HT2A Receptor antibody (ab66049)
    ab66049 at 1/100 dilution staining 5HT2A Receptor in Mouse frontal cortical neuron cultures (p1 pups, day 4 culture, for 48hrs) by Immunohistochemistry, Paraformaldehyde-fixed tissue.
  • Western blot - Anti-5HT2A Receptor antibody (ab66049)
    Western blot - Anti-5HT2A Receptor antibody (ab66049)
    Anti-5HT2A Receptor antibody (ab66049) at 1/400 dilution + Human Platelet (Human adult normal cell line) Whole Cell Lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 53 kDa
    Observed band size: 53 kDa
    Additional bands at: 57 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 4 minutes
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-5HT2A Receptor antibody (ab66049)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-5HT2A Receptor antibody (ab66049)
    ab66049, at 1/300 dilution staining 5HT2A Receptor in Rat cerebral cortex by Immunohistochemistry, Paraformaldehyde-fixed tissue.

References

This product has been referenced in:
  • Szlachta M  et al. Repeated Clozapine Increases the Level of Serotonin 5-HT1AR Heterodimerization with 5-HT2Aor Dopamine D2Receptors in the Mouse Cortex. Front Mol Neurosci 11:40 (2018). Read more (PubMed: 29497362) »
  • Wang P  et al. Inflammatory mediators mediate airway smooth muscle contraction through a G protein-coupled receptor-transmembrane protein 16A-voltage-dependent Ca2+ channel axis and contribute to bronchial hyperresponsiveness in asthma. J Allergy Clin Immunol 141:1259-1268.e11 (2018). WB . Read more (PubMed: 28754608) »

See all 7 Publications for this product

Publishing research using ab66049? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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5 reviews or Q&A

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This customer has purchased ab66049 (Anti-5HT2A Receptor antibody) and has conducted the wb several times with rat sample. The results show no major band and high background, therefore, he would like to ask for your help to modify his experiment step, ...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

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Last month I ordered two antibodies on June 25th from your company. Their Cat# are # ab21218(Lot No:GR24347) and ab66049 (Lot No:GR28433). My order number is xxx and account number is xxx . We prefer to be refunded.    I appreciate your help!

Thank you for contacting us.

Your credit note ID is 21936.

I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed ...

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What was the blocking buffer? 5% Non-fat milk, blocking for 2h. What were the antibodies incubated in? Plastic incubation box. What was the wash solution? TBS-T(0.1% Triton-100) What were the times and temperatures of each of these steps? 3 times , e...

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Thank you for your message and for providing this further information. I am sorry to hear these products did not work a stated on our datasheet. I appreciate the time you have spent on these experiments, and would like to offer a free of charge repl...

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Inquiry: I can not detect the signal using the antibodies above. My sample is rat brain. I extracted the protein using RIPA buffer and the ratio is 1mg:5ul. After that put on ice for one hour and votexed them every 10min. Then centrifuge them with 16,0...

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Thank you for contacting us.

I am very sorry to hear that you have been experiencing troubles when using our products. I would also like to thank you for providing me with details of you protocol.Our products are covered by Abpromise guara...

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To whom it may concern,
1. Order details:
- Batch number:
- Abcam product code: ab66049
- Antibody storage conditions (temperature/reconstitution etc) aliquot and stored at -20oC
2. Please describe the problem (high background,...

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Thank you for taking time to complete our questionnaire. I am sorry to hear that this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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