Anti-5HT2A Receptor antibody (ab66049)
Key features and details
- Rabbit polyclonal to 5HT2A Receptor
- Suitable for: IHC-FoFr
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-5HT2A Receptor antibody
See all 5HT2A Receptor primary antibodies -
Description
Rabbit polyclonal to 5HT2A Receptor -
Host species
Rabbit -
Tested applications
Suitable for: IHC-FoFrmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat 5HT2A Receptor aa 1-100 (N terminal).
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Positive control
- Rat cortex, amygdala and hippocampus tissue; mouse frontal cortical neuron cultures.
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General notes
Upon delivery dilute with phosphate buffer or Tris buffer at dilutions no higher than 1/10, aliquot and freeze at -15° C or lower. Antibody can be stored for up to six months if handled as described above.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 1% BSA, 99% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-FoFr |
1/300 - 1/500. Biotin/Streptavidin-HRP Technique.
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Notes |
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IHC-FoFr
1/300 - 1/500. Biotin/Streptavidin-HRP Technique. |
Target
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Function
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production. -
Sequence similarities
Belongs to the G-protein coupled receptor 1 family. -
Domain
The PDZ domain-binding motif is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4. -
Cellular localization
Cell membrane. Localizes to the post-synaptic thickening of axo-dendritic synapses. - Information by UniProt
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Database links
- Entrez Gene: 3356 Human
- Entrez Gene: 15558 Mouse
- Entrez Gene: 29595 Rat
- Omim: 182135 Human
- SwissProt: P28223 Human
- SwissProt: P35363 Mouse
- SwissProt: P14842 Rat
- Unigene: 654586 Human
see all -
Alternative names
- 5 HT 2 antibody
- 5 HT 2A antibody
- 5 HT2 receptor antibody
see all
Images
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ab66049 at 1/100 dilution staining 5HT2A Receptor in Mouse frontal cortical neuron cultures (p1 pups, day 4 culture, for 48hrs) by Immunohistochemistry, Paraformaldehyde-fixed tissue.
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ab66049, at 1/300 dilution staining 5HT2A Receptor in Rat cerebral cortex by Immunohistochemistry, Paraformaldehyde-fixed tissue.
Datasheets and documents
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Datasheet download
References (13)
ab66049 has been referenced in 13 publications.
- Ju A et al. Expression of serotonin 1A and 2A receptors in molecular- and projection-defined neurons of the mouse insular cortex. Mol Brain 13:99 (2020). PubMed: 32594910
- Gao W et al. HTR2A promotes the development of cardiac hypertrophy by activating PI3K-PDK1-AKT-mTOR signaling. Cell Stress Chaperones 25:899-908 (2020). PubMed: 32519137
- Eickelbeck D et al. CaMello-XR enables visualization and optogenetic control of Gq/11 signals and receptor trafficking in GPCR-specific domains. Commun Biol 2:60 (2019). PubMed: 30793039
- Delicata F et al. Preferential modulation of the lateral habenula activity by serotonin-2A rather than -2C receptors: Electrophysiological and neuroanatomical evidence. CNS Neurosci Ther 24:721-733 (2018). PubMed: 29479825
- Szlachta M et al. Repeated Clozapine Increases the Level of Serotonin 5-HT1AR Heterodimerization with 5-HT2Aor Dopamine D2Receptors in the Mouse Cortex. Front Mol Neurosci 11:40 (2018). PubMed: 29497362