Overview

  • Product name
    5HT2A Receptor peptide

Description

  • Nature
    Synthetic

Specifications

Our Abpromise guarantee covers the use of ab16195 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Form
    Liquid
  • Additional notes

    - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
    - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
    - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
    - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
    - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    Information available upon request.

General Info

  • Alternative names
    • 5 HT 2
    • 5 HT 2A
    • 5 HT2 receptor
    • 5 HT2A
    • 5 hydroxytryptamine receptor 2A
    • 5-HT-2
    • 5-HT-2A
    • 5-hydroxytryptamine (serotonin) receptor 2A, G protein-coupled
    • 5-hydroxytryptamine 2A receptor
    • 5-hydroxytryptamine receptor 2A
    • 5HT2A_HUMAN
    • HTR 2
    • HTR 2A
    • HTR2
    • HTR2, formerly
    • HTR2A
    • serotonin 5-HT-2 receptor, formerly
    • serotonin 5-HT-2A receptor
    • Serotonin receptor 2A
    see all
  • Function
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system. This receptor is involved in tracheal smooth muscle contraction, bronchoconstriction, and control of aldosterone production.
  • Sequence similarities
    Belongs to the G-protein coupled receptor 1 family.
  • Domain
    The PDZ domain-binding motif is involved in the interaction with INADL, CASK, APBA1, DLG1 and DLG4.
  • Cellular localization
    Cell membrane. Localizes to the post-synaptic thickening of axo-dendritic synapses.
  • Information by UniProt

References

ab16195 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thanks for your email.

Indeed, with corresponding antibody I meant ab16028.

The anti-5HT2A Receptor ab85496 may or may not detect the peptide ab16195, although to our knowledge this has not been tested yet.

The ab16195 peptide was derived from the N-terminus of the rat 5HT2A Receptor.

The antibody ab85496 detects an epitope within the peptide 'DILCEENTSLSSTTNSLMQLNDDTRLYSNDFNSGEANTSDAFNWTVDSEN' which is from the N-terminus of the human 5HT2A Receptor

The alignment of these sequences (see below) reveals that the corresponding sequences are quite similar. However, the exact epitope of ab85496 has not been mapped and we therefore do not know if the antibody would bind the corresponding region. Thus, ab85496 would detect ab16195 only if its epitope overlaps with the peptide (ab16195).

Unfortunately, the anti-5HT2A Receptor ab102703 would not detect the peptide ab16195 as the antibody was generated against a peptide derived from the C-terminus of the human 5HT2A.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your enquiry.
Indeed, it would be possible to detect the peptide ab16195 with the corresponding antibody on a Western blot. Given the small size of the peptide I would like torecommendoptimising the protocol conditions to ensure that the peptide is not running out of the gel and thatthe blotting step is efficient.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for contacting us.

Indeed, the 5HT2A Receptor peptide ab16195 can be used in studies with the anti-5HT2A Receptor antibody ab16028. The peptide is a 20mer and thus has a molecular weight of about 2.2 kDa. As ab16195 is the immunogen of the antibody it is meant to be used as blocking peptide, i.e. to block the specific signal of the antibody by preincubating to validate the results obtained.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Thank you for your inquiry.


No, you shouldn't have to strip the blot. I would wash 3 x 5 min in TBST, but then you should be all set to add the primary.


I hope this information helps. Please contact us with any other questions.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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