Overview

  • Product name

    Anti-5HT2C Receptor antibody [EPR6487]
    See all 5HT2C Receptor primary antibodies
  • Description

    Rabbit monoclonal [EPR6487] to 5HT2C Receptor
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human 5HT2C Receptor aa 400-500 (C terminal). The exact sequence is proprietary.
    Database link: P28335
    (Peptide available as ab170038)

  • Positive control

    • WB: SH-SY5Y whole cell lysate and human brain tissue lysate.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133570 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 52 kDa.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF,IHC-Fr,IHC-P or IP.
  • Target

    • Function

      This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
    • Sequence similarities

      Belongs to the G-protein coupled receptor 1 family.
    • Domain

      The PDZ domain-binding motif is involved in the interaction with MPDZ.
    • Post-translational
      modifications

      N-glycosylated.
    • Cellular localization

      Cell membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • 5 Hydroxytryptamine 2C receptor antibody
      • 5-HT-1C antibody
      • 5-ht-1c receptor antibody
      • 5-HT-2C antibody
      • 5-HT1C antibody
      • 5-HT2C antibody
      • 5-HTR2C antibody
      • 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled antibody
      • 5-hydroxytryptamine receptor 1C antibody
      • 5-hydroxytryptamine receptor 2C antibody
      • 5HT1C antibody
      • 5HT2C antibody
      • 5HT2C_HUMAN antibody
      • 5HTR2C antibody
      • 5Hydroxytryptamine 2C receptor antibody
      • Htr1c antibody
      • HTR2C antibody
      • serotonin 1c receptor antibody
      • serotonin 2c receptor antibody
      • Serotonin 5-HT-2C receptor antibody
      • Serotonin receptor 2C antibody
      see all

    Images

    • All lanes : Anti-5HT2C Receptor antibody [EPR6487] (ab133570) at 1/1000 dilution

      Lane 1 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
      Lane 2 : Human brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 52 kDa
      Observed band size: 75 kDa
      why is the actual band size different from the predicted?


      Exposure time: 3 minutes


      Blocking/Diluting buffer and concentration 5% NFDM/TBST

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    This product has been referenced in:

    • Nyarko JNK  et al. Glycosylation States of Pre- and Post-synaptic Markers of 5-HT Neurons Differ With Sex and 5-HTTLPR Genotype in Cortical Autopsy Samples. Front Neurosci 12:545 (2018). Read more (PubMed: 30147642) »
    • Ji G  et al. 5-HT2CReceptor Knockdown in the Amygdala Inhibits Neuropathic-Pain-Related Plasticity and Behaviors. J Neurosci 37:1378-1393 (2017). Read more (PubMed: 28011743) »
    See all 2 Publications for this product

    Customer reviews and Q&As

    1-5 of 5 Abreviews or Q&A

    Application
    Western blot
    Sample
    Rat Tissue lysate - whole (brain)
    Gel Running Conditions
    Reduced Denaturing (Bis-Tris 4-12%)
    Loading amount
    30 µg
    Specification
    brain
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

    Abcam user community

    Verified customer

    Submitted Jul 06 2016

    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Brain)
    Specification
    Brain
    Fixative
    Formaldehyde
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citric acid
    Permeabilization
    No
    Blocking step
    BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 21°C

    Mr. Carl Hobbs

    Verified customer

    Submitted Dec 04 2012

    Answer

    Thanks for your email.

    Abcam has recently aquired Epitomics so we list all Epitomics products on our catalog.I'm sorry for the confusion. I'm not sure of the reason for the discepency between the 2 molecular weights. I checked the SwissProt database and the theoretical MW of HTR2C is about 52kDa.

    I hope this is helpful. Please contact us again if you have any further questions.

    Read More
    This product is known to not work in this application or species.
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (brains 25um sections)
    Specification
    brains 25um sections
    Fixative
    formalin
    Permeabilization
    Yes - 1xPBT
    Blocking step
    BSA as blocking agent for 11 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 17°C

    Mr. Luke Burke

    Verified customer

    Submitted Sep 04 2012

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