Product nameAnti-5HT3A receptor antibody
See all 5HT3A receptor primary antibodies
DescriptionRabbit polyclonal to 5HT3A receptor
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human 5HT3A receptor aa 350-450 conjugated to keyhole limpet haemocyanin.
Database link: P46098
- This antibody gave a positive signal in the following human tissue lysates: Brain, Testis, Heart and Skeletal Muscle.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab45314 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 40, 55 kDa (predicted molecular weight: 55 kDa).|
|IHC-P||Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
Tissue specificityExpressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.
Cellular localizationCell junction, synapse, postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- 5 HT 3 antibody
- 5 HT3 receptor antibody
- 5 HT3A antibody
All lanes : Anti-5HT3A receptor antibody (ab45314) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Human testis tissue lysate - total protein (ab30257)
Lane 3 : Human heart tissue lysate - total protein (ab29431)
Lane 4 : Human skeletal muscle tissue lysate - total protein (ab29330)
Lysates/proteins at 10 µg per lane.
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 55 kDa
Observed band size: 40,55 kDa why is the actual band size different from the predicted?
In heart and skeletal muscle, ab45314 detects a band at 55 kDa, corresponding to the predicted molecular weight of 5HT3A. We are unsure as to why a lower band of 40 kDa is seen in brain and testis. However, subsequent re-testing of this antibody on testis produced a single band at 55 kDa, suggesting that the smaller band originally observed may be due to lysate variation. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
IHC image of 5HT3A staining in human hippocampus FFPE section, performed on a BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45314, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.