Anti-5HT3A receptor/5ht3 antibody (ab13897)

Reviews (6)Q&A (2)References (2)

Overview

  • Product name

    Anti-5HT3A receptor/5ht3 antibody
    See all 5HT3A receptor/5ht3 primary antibodies

  • Description

    Rabbit polyclonal to 5HT3A receptor/5ht3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide corresponding to Rat 5HT3A receptor/5ht3. The immunogen is a mixture of synthetic peptides corresponding to amino acids 27-42 (RATQAHSTTQPALLRL) and 427-442 (LSSIRHSLEKRDEMRE).
    Database link: P35563

  • Positive control

    • human brain lysate

  • General notes

     This product was previously labelled as 5HT3A receptor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab13897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 55 kDa.

Target

  • Function

    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.

  • Tissue specificity

    Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.

  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.

  • Cellular localization

    Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • 5 HT 3 antibody
    • 5 HT3 receptor antibody
    • 5 HT3A antibody
    • 5 HT3A receptor antibody
    • 5 HT3RA antibody
    • 5 hydroxytryptamine (serotonin) receptor 3A antibody
    • 5-HT-3 antibody
    • 5-HT3-A antibody
    • 5-HT3A antibody
    • 5-HT3R antibody
    • 5-hydroxytryptamine receptor 3 antibody
    • 5-hydroxytryptamine receptor 3A antibody
    • 5HT receptor antibody
    • 5HT3 serotonin receptor antibody
    • 5HT3A_HUMAN antibody
    • 5HT3R antibody
    • HTR3 antibody
    • Htr3a antibody
    • Serotonin receptor 3A antibody
    • Serotonin-gated ion channel receptor 3a antibody
    • Serotonin-gated ion channel receptor antibody
    see all

Images

  • Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)
    Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)
    All lanes : Anti-5HT3A receptor/5ht3 antibody (ab13897) at 3 µg/ml

    Lane 1 : Human Brain tissue lysate
    Lane 2 : Mouse Brain tissue lysate
    Lane 3 : Rat Brain tissue lysate

    Predicted band size: 55 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT3A receptor/5ht3 antibody (ab13897)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT3A receptor/5ht3 antibody (ab13897)
    IHC image of ab13897 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)
    Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)

    Western Blot of human brain lysate staining 5HT3A receptor with ab13897

References

This product has been referenced in:

  • Fu YL  et al. LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors. Biochem Biophys Res Commun 511:356-362 (2019). Read more (PubMed: 30791981) »
  • Nguyen H  et al. Downregulated hypothalamic 5-HT3 receptor expression and enhanced 5-HT3 receptor antagonist-mediated improvement in fatigue-like behaviour in cholestatic rats. Neurogastroenterol Motil 20:228-35 (2008). WB ; Rat . Read more (PubMed: 17919312) »
See all 2 Publications for this product

Publishing research using ab13897? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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1-8 of 8 Abreviews or Q&A

Immunohistochemistry (Frozen sections) abreview for Anti-5HT3A receptor/5ht3 antibody

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brain)
Permeabilization
Yes - 0.1% TritonX-100
Specification
brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Weihua Wang​

Verified customer

Submitted Apr 07 2017

Immunocytochemistry/ Immunofluorescence abreview for Anti-5HT3A receptor antibody

 Average
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Cell (SH-SY5Y))
Permeabilization
Yes - TRITON X-100 (0'25%)
Specification
Human Cell (SH-SY5Y)
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Dec 06 2016

Immunohistochemistry (Frozen sections) abreview for Anti-5HT3A receptor/5ht3 antibody

 Average
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue (Brain)
Permeabilization
No
Specification
Brain
Blocking step
Blocking Buffer as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted Sep 25 2014

Western blot abreview for Anti-5HT3A receptor antibody

 Good
Abreviews
abreview image
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing (10% SDS-acryamide gel)
Sample
Rat Tissue lysate - whole (rat-colon)
Specification
rat-colon
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 28°C
Read More

Abcam user community

Verified customer

Submitted Sep 22 2014

Immunohistochemistry (Frozen sections) abreview for Anti-5HT3A receptor antibody

 Inconclusive
Abreviews
Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample
Mouse Tissue sections (tongue, taste buds)
Specification
tongue, taste buds
Permeabilization
Yes - 0.3 % Triton, and 1 % Triton
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

Submitted May 02 2014

Question

From your description of antibody ab13897:
Synthetic peptides, corresponding to amino acids 21-36 and 421-436 of Rat 5HT3 Receptor

Do the numbers correspond to the protein sequence with or without the signal peptide? I am trying to estimate whether it might react with xenopus.

Read More
Answer

Thank you for contacting Abcam.

The amino acids correspond to the protein sequence with the signal peptide (SwissProt: P35563). We normally look for at least 85% degree of homology before we would recommend using an antibody in an untested species. I cannot find the Xenopus sequence, but if you would like to send it to me, i will be happy to do the alignment and see if there is a good chance that the antibody would work.

If there is anything else I can help you with, please let me know.

Read More

Question

IHC-P image legend says "detected using an HRP conjugated compact polymer system" Which HRP conjugated compact polymer system was used?

Read More
Answer

Thank you for contacting us. The detection kit we used for the IHC image is the Leica Bond refine polymer detection kit (for the automated Leica Bond IHC system). The system uses small linkers to enhance and refine the detection of the target protein, linked through a compact polymer backbone. The kit contains a peroxide block, a post primary, polymer, DAB chromogen and a haematoxylin counterstain. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Western blot abreview for Anti-5HT3A receptor/5ht3 antibody

 Average
Abreviews
Application
Western blot
Sample
Rat Cell lysate - whole cell (rat cerebellar homogenate)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Treatment
control and 20 mg/kg cocaine
Specification
rat cerebellar homogenate
Blocking step
Commercially available blocking agent as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0.2% · Temperature: 20°C
Read More

Mr. Jean-Bernard Dietrich

Verified customer

Submitted Sep 20 2007

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