Anti-5HT3A receptor/5ht3 antibody (ab13897)
Key features and details
- Rabbit polyclonal to 5HT3A receptor/5ht3
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-5HT3A receptor/5ht3 antibody
See all 5HT3A receptor/5ht3 primary antibodies -
Description
Rabbit polyclonal to 5HT3A receptor/5ht3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Rat 5HT3A receptor/5ht3. The immunogen is a mixture of synthetic peptides corresponding to amino acids 27-42 (RATQAHSTTQPALLRL) and 427-442 (LSSIRHSLEKRDEMRE).
Database link: P35563 -
Positive control
- human brain lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13897 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| WB | (2) |
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 55 kDa.
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| Notes |
|---|
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IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
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WB
Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 55 kDa. |
Target
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Function
This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel. -
Tissue specificity
Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta. -
Sequence similarities
Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily. -
Cellular localization
Cell junction, synapse, postsynaptic cell membrane. Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 3359 Human
- Entrez Gene: 15561 Mouse
- Entrez Gene: 79246 Rat
- Omim: 182139 Human
- SwissProt: P46098 Human
- SwissProt: P23979 Mouse
- SwissProt: P35563 Rat
- Unigene: 413899 Human
see all -
Alternative names
- 5 HT 3 antibody
- 5 HT3 receptor antibody
- 5 HT3A antibody
see all
Images
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Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)All lanes : Anti-5HT3A receptor/5ht3 antibody (ab13897) at 3 µg/ml
Lane 1 : Human Brain tissue lysate
Lane 2 : Mouse Brain tissue lysate
Lane 3 : Rat Brain tissue lysate
Predicted band size: 55 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT3A receptor/5ht3 antibody (ab13897)IHC image of ab13897 staining in human normal cerebral cortex formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13897, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times. -
Western blot - Anti-5HT3A receptor/5ht3 antibody (ab13897)Western Blot of human brain lysate staining 5HT3A receptor with ab13897
Protocols
Datasheets and documents
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Datasheet download
References (6)
ab13897 has been referenced in 6 publications.
- Zhu Y et al. Effects of sacral nerve electrical stimulation on 5-HT and 5-HT3AR/5-HT4R levels in the colon and sacral cord of acute spinal cord injury rat models. Mol Med Rep 22:763-773 (2020). PubMed: 32626986
- Cheng Q et al. [6]-Gingerol Ameliorates Cisplatin-Induced Pica by Regulating the TPH/MAO-A/SERT/5-HT/5-HT3 Receptor System in Rats. Drug Des Devel Ther 14:4085-4099 (2020). PubMed: 33061309
- Fu YL et al. LMAN1 (ERGIC-53) promotes trafficking of neuroreceptors. Biochem Biophys Res Commun 511:356-362 (2019). PubMed: 30791981
- Li B et al. Deciphering the multicomponent synergy mechanisms of SiNiSan prescription on irritable bowel syndrome using a bioinformatics/network topology based strategy. Phytomedicine 63:152982 (2019). PubMed: 31299593
- Zhao L et al. Prolongation of bronchopulmonary C-fiber-mediated apnea by prenatal nicotinic exposure in rat pups: role of 5-HT3 receptors. FASEB J 33:10731-10741 (2019). PubMed: 31251077
- Nguyen H et al. Downregulated hypothalamic 5-HT3 receptor expression and enhanced 5-HT3 receptor antagonist-mediated improvement in fatigue-like behaviour in cholestatic rats. Neurogastroenterol Motil 20:228-35 (2008). WB ; Rat . PubMed: 17919312
