Overview

  • Product name

    Anti-5HT3A receptor/5ht3 antibody
    See all 5HT3A receptor/5ht3 primary antibodies
  • Description

    Goat polyclonal to 5HT3A receptor/5ht3
  • Host species

    Goat
  • Specificity

    ab51950 is expected to recognise both reported isoforms (NP_998786.1 and NP_000860.1).
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human 5HT3A receptor/5ht3 aa 390-401 (internal sequence).
    Sequence:

    CGPQDFEKSPRDR


    Database link: P46098

  • Positive control

    • Human Colon lysates
  • General notes

     This product was previously labelled as 5HT3A receptor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab51950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa). A band of unknown identity is also consistently observed at 200kDa. This band is successfully blocked by incubation with the immunising peptide but we are unsure of it's identity.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
  • Tissue specificity

    Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.
  • Cellular localization

    Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5 HT 3 antibody
    • 5 HT3 receptor antibody
    • 5 HT3A antibody
    • 5 HT3A receptor antibody
    • 5 HT3RA antibody
    • 5 hydroxytryptamine (serotonin) receptor 3A antibody
    • 5-HT-3 antibody
    • 5-HT3-A antibody
    • 5-HT3A antibody
    • 5-HT3R antibody
    • 5-hydroxytryptamine receptor 3 antibody
    • 5-hydroxytryptamine receptor 3A antibody
    • 5HT receptor antibody
    • 5HT3 serotonin receptor antibody
    • 5HT3A_HUMAN antibody
    • 5HT3R antibody
    • HTR3 antibody
    • Htr3a antibody
    • Serotonin receptor 3A antibody
    • Serotonin-gated ion channel receptor 3a antibody
    • Serotonin-gated ion channel receptor antibody
    see all

Images

  • Anti-5HT3A receptor/5ht3 antibody (ab51950) at 0.3 µg/ml + 35 µg of Human Colon lysate (in RIPA buffer)

    Predicted band size: 55 kDa
    Observed band size: 55 kDa
    Additional bands at: 200 kDa. We are unsure as to the identity of these extra bands.

References

This product has been referenced in:

  • Li F  et al. Shuyu Capsules Relieve Premenstrual Syndrome Depression by Reducing 5-HT3AR and 5-HT3BR Expression in the Rat Brain. Neural Plast 2016:7950781 (2016). Read more (PubMed: 27725889) »
See 1 Publication for this product

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