• Product name

    Anti-5HT3A receptor/5ht3 antibody
    See all 5HT3A receptor/5ht3 primary antibodies
  • Description

    Rabbit polyclonal to 5HT3A receptor/5ht3
  • Host species

  • Specificity

    5-HT-3A Antibody detects endogenous levels of total 5-HT-3A protein.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human 5HT3A receptor/5ht3 aa 161-210 (internal sequence).
    Database link: P46098

  • Positive control

    • Extracts from HT-29 cells
  • General notes

     This product was previously labelled as 5HT3A receptor




Our Abpromise guarantee covers the use of ab69596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA 1/10000.


  • Function

    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
  • Tissue specificity

    Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.
  • Cellular localization

    Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5 HT 3 antibody
    • 5 HT3 receptor antibody
    • 5 HT3A antibody
    • 5 HT3A receptor antibody
    • 5 HT3RA antibody
    • 5 hydroxytryptamine (serotonin) receptor 3A antibody
    • 5-HT-3 antibody
    • 5-HT3-A antibody
    • 5-HT3A antibody
    • 5-HT3R antibody
    • 5-hydroxytryptamine receptor 3 antibody
    • 5-hydroxytryptamine receptor 3A antibody
    • 5HT receptor antibody
    • 5HT3 serotonin receptor antibody
    • 5HT3A_HUMAN antibody
    • 5HT3R antibody
    • HTR3 antibody
    • Htr3a antibody
    • Serotonin receptor 3A antibody
    • Serotonin-gated ion channel receptor 3a antibody
    • Serotonin-gated ion channel receptor antibody
    see all


  • All lanes : Anti-5HT3A receptor/5ht3 antibody (ab69596) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells
    Lane 2 : Extracts from HT-29 cells with the immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa
    Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.


ab69596 has not yet been referenced specifically in any publications.

Customer reviews and Q&As


Thank you very much for your inquiry.

If I understand your customers plans correctly, they are looking for an antibody that can block the binding site of the receptor which would be a functional application.

According the the Swiss Prot homepage the mutation of amino acid 178 1 W → S abolished ligand binding to the heteromeric receptor. Ref.Characterization of the novel human serotonin receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E."
Niesler B., Walstab J., Combrink S., Moeller D., Kapeller J., Rietdorf J., Boenisch H., Goethert M., Rappold G., Bruess M.
Mol. Pharmacol. 72:8-17(2007) [PubMed: 17392525]

Unfortunately, none of our antibodies bind at this site and therefore I do not have an antibody that I can recommend for testing on this occasion.

I am sorry I did not have a positive answer for your customer and hope this information is nevertheless helpful.

Merry Christmas to you and your customer!

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