Key features and details
- Rabbit polyclonal to 5HT3A receptor/5ht3
- Suitable for: WB, ELISA
- Reacts with: Human
- Isotype: IgG
Product nameAnti-5HT3A receptor/5ht3 antibody
See all 5HT3A receptor/5ht3 primary antibodies
DescriptionRabbit polyclonal to 5HT3A receptor/5ht3
Specificity5-HT-3A Antibody detects endogenous levels of total 5-HT-3A protein.
Tested applicationsSuitable for: WB, ELISAmore details
Species reactivityReacts with: Human
Synthetic peptide corresponding to Human 5HT3A receptor/5ht3 aa 161-210 (internal sequence).
Database link: P46098
- Extracts from HT-29 cells
This product was previously labelled as 5HT3A receptor
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Purification notesThe antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
Our Abpromise guarantee covers the use of ab69596 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).|
FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
Tissue specificityExpressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.
Sequence similaritiesBelongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.
Cellular localizationCell junction, synapse, postsynaptic cell membrane. Cell membrane.
- Information by UniProt
- 5 HT 3 antibody
- 5 HT3 receptor antibody
- 5 HT3A antibody
All lanes : Anti-5HT3A receptor/5ht3 antibody (ab69596) at 1/500 dilution
Lane 1 : Extracts from HT-29 cells
Lane 2 : Extracts from HT-29 cells with the immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 55 kDa
Observed band size: 55 kDa
Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.
ab69596 has not yet been referenced specifically in any publications.