Overview

  • Product name

    Anti-5HT3A receptor/5ht3 antibody
    See all 5HT3A receptor/5ht3 primary antibodies
  • Description

    Rabbit polyclonal to 5HT3A receptor/5ht3
  • Host species

    Rabbit
  • Specificity

    5-HT-3A Antibody detects endogenous levels of total 5-HT-3A protein.
  • Tested applications

    Suitable for: WB, ELISAmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human 5HT3A receptor/5ht3 aa 161-210 (internal sequence).
    Database link: P46098

  • Positive control

    • Extracts from HT-29 cells
  • General notes

     This product was previously labelled as 5HT3A receptor

     

Properties

Applications

Our Abpromise guarantee covers the use of ab69596 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
ELISA 1/10000.

Target

  • Function

    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses in neurons. It is a cation-specific, but otherwise relatively nonselective, ion channel.
  • Tissue specificity

    Expressed in cerebral cortex, amygdala, hippocampus, and testis. Detected in monocytes of the spleen and tonsil, in small and large intestine, uterus, prostate, ovary and placenta.
  • Sequence similarities

    Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3A sub-subfamily.
  • Cellular localization

    Cell junction, synapse, postsynaptic cell membrane. Cell membrane.
  • Information by UniProt
  • Database links

  • Alternative names

    • 5 HT 3 antibody
    • 5 HT3 receptor antibody
    • 5 HT3A antibody
    • 5 HT3A receptor antibody
    • 5 HT3RA antibody
    • 5 hydroxytryptamine (serotonin) receptor 3A antibody
    • 5-HT-3 antibody
    • 5-HT3-A antibody
    • 5-HT3A antibody
    • 5-HT3R antibody
    • 5-hydroxytryptamine receptor 3 antibody
    • 5-hydroxytryptamine receptor 3A antibody
    • 5HT receptor antibody
    • 5HT3 serotonin receptor antibody
    • 5HT3A_HUMAN antibody
    • 5HT3R antibody
    • HTR3 antibody
    • Htr3a antibody
    • Serotonin receptor 3A antibody
    • Serotonin-gated ion channel receptor 3a antibody
    • Serotonin-gated ion channel receptor antibody
    see all

Images

  • All lanes : Anti-5HT3A receptor/5ht3 antibody (ab69596) at 1/500 dilution

    Lane 1 : Extracts from HT-29 cells
    Lane 2 : Extracts from HT-29 cells with the immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 55 kDa
    Observed band size: 55 kDa
    Additional bands at: 53 kDa. We are unsure as to the identity of these extra bands.

References

ab69596 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you very much for your inquiry.

If I understand your customers plans correctly, they are looking for an antibody that can block the binding site of the receptor which would be a functional application.

According the the Swiss Prot homepage the mutation of amino acid 178 1 W → S abolished ligand binding to the heteromeric receptor. Ref.Characterization of the novel human serotonin receptor subunits 5-HT3C, 5-HT3D, and 5-HT3E."
Niesler B., Walstab J., Combrink S., Moeller D., Kapeller J., Rietdorf J., Boenisch H., Goethert M., Rappold G., Bruess M.
Mol. Pharmacol. 72:8-17(2007) [PubMed: 17392525]

Unfortunately, none of our antibodies bind at this site and therefore I do not have an antibody that I can recommend for testing on this occasion.

I am sorry I did not have a positive answer for your customer and hope this information is nevertheless helpful.

Merry Christmas to you and your customer!

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