Overview

  • Product name
    Anti-5HT3B receptor antibody
    See all 5HT3B receptor primary antibodies
  • Description
    Rabbit polyclonal to 5HT3B receptor
  • Host species
    Rabbit
  • Specificity
    Specific to 5HT3B - does not recognise 5HT3A receptor This antibody recognizes the intracellular region of the 5HT3B subunit.
  • Tested applications
    Suitable for: ELISA, WB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide, corresponding to amino acids 320-338 of Human 5HT3B receptor (large intracellular loop region)

Properties

Applications

Our Abpromise guarantee covers the use of ab39629 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration.
WB 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
IHC-P Use at an assay dependent concentration.
ICC/IF 1/500.
IP Use at an assay dependent concentration.

Target

  • Function
    This is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor is a ligand-gated ion channel, which when activated causes fast, depolarizing responses. It is a cation-specific, but otherwise relatively nonselective, ion channel.
  • Tissue specificity
    Expressed in the brain cortex, in the caudate nucleus, the hyppocampus, the thalamus and the amygdala. Detected in the kidney and testis as well as in monocytes of the spleen, small and large intestine, uterus, prostate, ovary and placenta.
  • Sequence similarities
    Belongs to the ligand-gated ion channel (TC 1.A.9) family. 5-hydroxytryptamine receptor (TC 1.A.9.2) subfamily. HTR3B sub-subfamily.
  • Post-translational
    modifications
    N-glycosylation required for membrane localization.
  • Cellular localization
    Cell membrane. Presumably retained within the endoplasmic reticulum unless complexed with HTR3A.
  • Information by UniProt
  • Database links
  • Alternative names
    • 5 HT3 B antibody
    • 5 HT3B antibody
    • 5 HTR3B antibody
    • 5 hydroxytryptamine 3 receptor B subunit antibody
    • 5 hydroxytryptamine receptor 3B antibody
    • 5 hydroxytryptamine serotonin receptor 3B antibody
    • 5-HT3-B antibody
    • 5-HT3B antibody
    • 5-hydroxytryptamine receptor 3B antibody
    • 5HT3B_HUMAN antibody
    • 5HTR3B antibody
    • Htr3b antibody
    • Serotonin gated ion channel subunit antibody
    • Serotonin receptor 3B antibody
    see all

Images

  • All lanes : Anti-5HT3B receptor antibody (ab39629) at 1/500 dilution

    Lane 1 : Lysate from HEK 293 cells
    Lane 2 : Lysate from HEK 293 cells transfected with 5HT3A receptor
    Lane 3 : Lysate from HEK 293 cells transfected with 5HT3A receptor and 5HT3B receptor
    Lane 4 : Human hippocampus lysate

    Predicted band size: 50 kDa
    Observed band size: 50 kDa



    Pre-incubation of ab39629 with its immunizing peptide (at 10uM) results in a loss of immunoreactivity (lower panel).
  • HEK 293 cells transfected with 5HT3B receptor were stained with ab39629 diluted 1/500 and anti-rabbit secondary antibody conjugated to Alexa Fluor 488 diluted 1/1000(green). Nuclei are labelled with Hoescht 33258 (blue).
  • Immunohistochemistry of human hippocampus stained with ab39269 diluted 1/500. Immunoreactivity with 5HT3B receptor can be seen in (i)CA1 field, (ii)CA2, (iii)CA3 and (iv)CA4/dentate gyrus.

References

This product has been referenced in:
  • Babic T  et al. Glucose-dependent trafficking of 5-HT3 receptors in rat gastrointestinal vagal afferent neurons. Neurogastroenterol Motil 24:e476-88 (2012). Read more (PubMed: 22845622) »
  • Brady CA  et al. Identification of 5-HT(3A) and 5-HT(3B) receptor subunits in human hippocampus. Neuropharmacology : (2007). Read more (PubMed: 17327132) »
See all 2 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Question
Answer

Thank you very much for your interest in ab39629.
To our knowledge, ab39629 has not been tested in flow cytometry. Therefore, I can offer a discount off a future purchase if you buy ab39629 now, test it in flow cytometry and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody.
If you are interested in this offer, please follow these steps:
1. Reply to this e-mail to let me know that you would like to proceed and test ab39629 in flow cytometry. I will then send a discount code. This code must be issued before purchasing ab39629 so please wait for my reply before ordering.
2. Purchase ab39629 either by phone, fax, or online (www.abcam.com).
3. Test it in flow cytometry.
4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.
5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.
We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab39629 turns out to be unsuitable for flow cytometry, you will still receive the discount on your next purchase after your Abreview has been submitted.
Please let me know if you have any questions about this offer and I would be happy to help you further.
The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you for contacting us. We would normally recommend using a rabbit IgG and use the same donkey anti-rabbit FITC secondary in order to detect any interaction. Your Rabbit IgG Isotype control-FITC should be applicable but may cause some differences in the experimental conditions such as the level of FITC between the two cases may be different and you would not detect any non-specific interaction that may be coming from the interaction of the secondary antibody directly. I would therefore make sure that the concentrations of the primary antibodies used are the same and that a "no primary" control is also performed. I hope this information has been of help. If you have any further questions, please do not hesitate to ask.

Read More

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up