Anti-5HT4 Receptor antibody (ab60359)
Key features and details
- Rabbit polyclonal to 5HT4 Receptor
- Suitable for: WB, ICC/IF
- Reacts with: Human, Zebrafish
- Isotype: IgG
Overview
-
Product name
Anti-5HT4 Receptor antibody
See all 5HT4 Receptor primary antibodies -
Description
Rabbit polyclonal to 5HT4 Receptor -
Host species
Rabbit -
Specificity
5-HT-4 antibody detects endogenous levels of total 5-HT-4 protein. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human, Zebrafish
Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Opossum
-
Immunogen
Synthetic peptide corresponding to Human 5HT4 Receptor aa 21-70.
Sequence:LMAILGNLLV MVAVCWDRQL RKIKTNYFIV SLAFADLLVS
Database link: Q13639 -
Positive control
- WB: Jurkat cell, COLO205 cells. ICC: HeLa cells.
-
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab60359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/1000. Predicted molecular weight: 44 kDa.
|
|
| ICC/IF |
1/500 - 1/1000.
|
| Notes |
|---|
|
WB
1/500 - 1/1000. Predicted molecular weight: 44 kDa. |
|
ICC/IF
1/500 - 1/1000. |
Target
-
Relevance
The 5HT4 Receptor is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulates adenylate cyclase. 5HT4a receptors may play a role in opioid-induced respiratory depression. -
Cellular localization
Cell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli. -
Database links
- Entrez Gene: 317708 Cow
- Entrez Gene: 100135548 Guinea pig
- Entrez Gene: 100034053 Horse
- Entrez Gene: 3360 Human
- Entrez Gene: 15562 Mouse
- Entrez Gene: 397431 Pig
- Entrez Gene: 25324 Rat
- Entrez Gene: 710046 Rhesus monkey
see all -
Alternative names
- 5 HT 4 antibody
- 5 HT4 antibody
- 5 HT4R antibody
see all
Images
-
Western blot - Anti-5HT4 Receptor antibody (ab60359)All lanes : Anti-5HT4 Receptor antibody (ab60359)
Lane 1 : Jurkat cell lysate
Lane 2 : COLO205 cell lysate
Lane 3 : COLO205 cell lysate with Synthesized peptide
Predicted band size: 44 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-5HT4 Receptor antibody (ab60359)ab60359, staining 5-HT-4 receptor in HeLa cells by ICC/IF (left), with blocking peptide (right). -
Western blot - Anti-5HT4 Receptor antibody (ab60359)Anti-5HT4 Receptor antibody (ab60359) at 1/1000 dilution + COLO205 cells
Predicted band size: 44 kDa -
Western blot - Anti-5HT4 Receptor antibody (ab60359)All lanes : Anti-5HT4 Receptor antibody (ab60359) at 1/500 dilution
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 kDa why is the actual band size different from the predicted?
Exposure time: 5 minutes
Protocols
Datasheets and documents
-
Datasheet download
References (4)
ab60359 has been referenced in 4 publications.
- Zhu Y et al. Effects of sacral nerve electrical stimulation on 5-HT and 5-HT3AR/5-HT4R levels in the colon and sacral cord of acute spinal cord injury rat models. Mol Med Rep 22:763-773 (2020). PubMed: 32626986
- El-Nachef WN & Bronner ME De novo enteric neurogenesis in post-embryonic zebrafish from Schwann cell precursors rather than resident cell types. Development 147:N/A (2020). PubMed: 32541008
- Cavaccini A et al. Serotonergic Signaling Controls Input-Specific Synaptic Plasticity at Striatal Circuits. Neuron 98:801-816.e7 (2018). PubMed: 29706583
- Hodge E et al. HTR4 gene structure and altered expression in the developing lung. Respir Res 14:77 (2013). IHC . PubMed: 23890215
