- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-5HT4 Receptor antibody
See all 5HT4 Receptor primary antibodies -
DescriptionRabbit polyclonal to 5HT4 Receptor
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Host speciesRabbit
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Specificity5-HT-4 antibody detects endogenous levels of total 5-HT-4 protein.
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Tested applicationsSuitable for: IHC-P, WB, ICC/IF, ELISAmore details
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Species reactivityReacts with: Human, Zebrafish
Predicted to work with: Mouse, Rat, Horse, Chicken, Guinea pig, Cow, Pig, Chimpanzee, Rhesus monkey, Opossum
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Immunogen
Synthetic peptide: LMAILGNLLV MVAVCWDRQ L RKIKTNYF IV SLAFADL LVS, corresponding to amino acids 31/70 of Human 5HT4 Receptor
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Positive control
- 293 cells, treated with insulin (0.01U/ml, 15mins)
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
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Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride -
Concentration information loading... -
PurityImmunogen affinity purified
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab60359 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use at an assay dependent concentration. | |
| WB | 1/500 - 1/1000. Predicted molecular weight: 44 kDa. | |
| ICC/IF | 1/500 - 1/1000. | |
| ELISA | 1/5000. |
Target
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RelevanceThe 5HT4 Receptor is one of the several different receptors for 5- hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulates adenylate cyclase. 5HT4a receptors may play a role in opioid-induced respiratory depression.
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Cellular localizationCell membrane; Multi-pass membrane protein. Endosome. Note: Interaction with SNX27 mediates recruitment to early endosomes, while interaction with SLC9A3R1 and EZR might target the protein to specialized subcellular regions, such as microvilli.
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Database links
- Entrez Gene: 317708 Cow
- Entrez Gene: 100135548 Guinea pig
- Entrez Gene: 100034053 Horse
- Entrez Gene: 3360 Human
- Entrez Gene: 15562 Mouse
- Entrez Gene: 397431 Pig
- Entrez Gene: 25324 Rat
- Entrez Gene: 710046 Rhesus monkey
see all -
Alternative names
- 5 HT 4 antibody
- 5 HT4 antibody
- 5 HT4R antibody
see all
Images
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Western blot - Anti-5HT4 Receptor antibody (ab60359)All lanes : Anti-5HT4 Receptor antibody (ab60359)
Lane 1 : 293 cells treated with insulin (0.01U/ml, 15 mins)
Lane 2 : 293 cells treated with insulin (0.01U/ml, 15 mins) with blocking peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 44 kDa -
Immunocytochemistry/ Immunofluorescence - Anti-5HT4 Receptor antibody (ab60359)ab60359, staining 5-HT-4 receptor in HeLa cells by ICC/IF (left), with blocking peptide (right). -
Western blot - Anti-5HT4 Receptor antibody (ab60359)All lanes : Anti-5HT4 Receptor antibody (ab60359) at 1/500 dilution
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Secondary
All lanes : Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 44 kDa
Observed band size: 42 kDa (why is the actual band size different from the predicted?)
Exposure time: 5 minutes
Protocols
References
This product has been referenced in:
- Hodge E et al. HTR4 gene structure and altered expression in the developing lung. Respir Res 14:77 (2013). IHC . Read more (PubMed: 23890215) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"