Key features and details
- Rabbit polyclonal to 5HT6 Receptor
- Suitable for: IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-5HT6 Receptor antibody
See all 5HT6 Receptor primary antibodies
DescriptionRabbit polyclonal to 5HT6 Receptor
Tested applicationsSuitable for: IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human 5HT6 Receptor aa 1-50. The exact sequence is proprietary. (NP_000862.1).
Database link: P50406
- IP: Jurkat whole cell lysate.
ab241473 has not performed satisfactorily when used for WB of 5HT6 Receptor in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of 5HT6 Receptor.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241473 was affinity purified using an epitope specific to 5HT6 Receptor immobilized on solid support.
Our Abpromise guarantee covers the use of ab241473 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-10 µg/mg of lysate.|
FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase. It has a high affinity for tricyclic psychotropic drugs.
Tissue specificityExpressed in several human brain regions, most prominently in the caudate nucleus.
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
Cellular localizationCell membrane.
- Information by UniProt
- 5 HT 6 antibody
- 5 HT6 antibody
- 5 HT6R antibody
5HT6 Receptor was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab241473 at 6 µg/reaction. Western blot was performed from the immunoprecipitate using ab241473 at 1 µg/ml.
Lane 1: ab241473 IP in Jurkat whole cell lysate.
Lane 2: Control IgG IP in Jurkat whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
Lysates prepared using RIPA lysis buffer.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab241473 has not yet been referenced specifically in any publications.