- Datasheet
- Specific References
- Protocols
Overview
-
Product nameAnti-5HT7 Receptor antibody
See all 5HT7 Receptor primary antibodies -
DescriptionRabbit polyclonal to 5HT7 Receptor
-
Host speciesRabbit
-
Tested applicationsSuitable for: IHC-P, ICC/IFmore details
-
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Hamster, Cow, Dog, Pig, Monkey, Gorilla, Common marmoset, Bat
-
Immunogen
Synthetic peptide conjugated to KLH, corresponding to 19 amino acids from the 3rd cytoplasmic domain of Human 5HT7 Receptor.
-
Positive control
- Human brain cortex tissue.
Properties
-
FormLiquid
-
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
-
Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS, pH 7.7 -
Concentration information loading... -
PurityImmunogen affinity purified
-
ClonalityPolyclonal
-
IsotypeIgG
-
Research areas
Associated products
-
Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab61562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/200. |
Target
-
FunctionThis is one of the several different receptors for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. The activity of this receptor is mediated by G proteins that stimulate adenylate cyclase.
-
Tissue specificityIsoform A is the predominant isoform in spleen, caudate and hippocampus. Isoform B is expressed at lower levels. Isoform D is a minor isoform in term of expression.
-
Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
-
Cellular localizationCell membrane.
- Information by UniProt
-
Database links
- Entrez Gene: 100135549 Guinea pig
- Entrez Gene: 3363 Human
- Entrez Gene: 15566 Mouse
- Entrez Gene: 65032 Rat
- Omim: 182137 Human
- SwissProt: P50407 Guinea pig
- SwissProt: P34969 Human
- SwissProt: P32304 Mouse
see all -
Alternative names
- 5 HT 7 antibody
- 5 HT X antibody
- 5 hydroxytryptamine (serotonin) receptor 7 (adenylate cyclase coupled) antibody
see all
Images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-5HT7 Receptor antibody (ab61562)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human brain cortex tissue, staining 5HT7 Receptor with ab61562 at 10 µg/ml. -
Immunocytochemistry/ Immunofluorescence - Anti-5HT7 Receptor antibody (ab61562)ICC/IF image of ab61562 stained SKNSH cells. The cells were 4% PFA fixed (10mins) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab61562, 1:200) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References
ab61562 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"