Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [B3F1] to 5T4
- Suitable for: Flow Cyt
- Reacts with: Mouse
Product nameAnti-5T4 antibody [B3F1]
See all 5T4 primary antibodies
DescriptionMouse monoclonal [B3F1] to 5T4
Tested applicationsSuitable for: Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or WB
Species reactivityReacts with: Mouse
- Flow Cyt: NIH/3T3 cells.
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact email@example.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab254168 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use a concentration of 1.012 mg/ml.|
Tissue specificityExpressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
Sequence similaritiesContains 6 LRR (leucine-rich) repeats.
Contains 1 LRRCT domain.
Contains 1 LRRNT domain.
- Information by UniProt
Form5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1–60 (Ward et al., 2006).
- 5T4 antibody
- 5T4 oncofetal antigen antibody
- 5T4 oncofetal trophoblast glycoprotein antibody
Flow cytometric analysis of RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage, Left) / NIH/3T3 (Mouse embryonic fibroblast, Right) labeling 5T4 with ab254168 at 1.012µg/ml (red) compared with Mouse monoclonal IgG Isotype Control (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Mouse IgG (Alexa Fluor® 488) (ab150113) at 1/2000 dilution was used as the secondary antibody.
Negative control: RAW 264.7 (PMID: 10366710).
Gated on viable cells.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab254168 has been referenced in 1 publication.
- Southgate TD et al. CXCR4 mediated chemotaxis is regulated by 5T4 oncofetal glycoprotein in mouse embryonic cells. PLoS One 5:e9982 (2010). PubMed: 20376365