Question (71953) | Anti-5T4 antibody [EPR5529] (ab134162)

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Question

Could I get the details of your pervanadate treatment in the WB image on the datasheet? The concentration, incubation time, and method of reconstituting would be very helpful.

Answer

Thank you for contacting us. Please find below the notes on the image with pervanadate.


Prepare 500mM stock Sodium Orthovanadate in PBS. To make
100X Pervanadate right before experiment, add 400ul 500mM Sodium Orthovanadate to 1560ul H2O and then 40ul H2O2(Fisher #H325-100), vortex and incubate at RT for 5-10min.

Cells are serum-starved for overnight, and then treated with freshly made Pervanadate for 20min at 37 degrees C.


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