Question (75004) | Anti-5T4 antibody [EPR5529] (ab134162)

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Question

when an antibody is not recommended for IHC, have you tested that product for all applications or is it sometimes that IHC simply wasn't tested?

if you're looking to do IHC with said antibody high pretreatment temp and pressure seem to help.

Answer



When an antibody is noted as 'unsuitable', it has been tested in that application. It may be that optimized conditions or samples were not used during this QC so I am happy to see that you have had success with your IHC. Thanks very much for your protocol advice. I will pass this on!

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