Recombinant
RabMAb

Recombinant Anti-5T4 antibody [EPR5529] - BSA and Azide free (ab201711)

Overview

  • Product name

    Anti-5T4 antibody [EPR5529] - BSA and Azide free
    See all 5T4 primary antibodies
  • Description

    Rabbit monoclonal [EPR5529] to 5T4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human 5T4 aa 100-200 (extracellular). The exact sequence is proprietary.
    Database link: Q13641

  • General notes

    The formulation and the concentration of this product is compatible for metal-conjugation for mass cytometry (CyTOF®).

    ab201711 is a PBS-only buffer format of ab134162. Please refer to ab134162 for recommended dilutions, protocols, and image data. Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information. 

     

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab201711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Predicted molecular weight: 46 kDa.
IHC-P Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Tissue specificity

      Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
    • Sequence similarities

      Contains 6 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Form

      5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1–60 (Ward et al., 2006).
    • Alternative names

      • 5T4 antibody
      • 5T4 oncofetal antigen antibody
      • 5T4 oncofetal trophoblast glycoprotein antibody
      • 5T4 oncotrophoblast glycoprotein antibody
      • 5T4AG antibody
      • AW495680 antibody
      • M6P1 antibody
      • TPBG antibody
      • TPBG_HUMAN antibody
      • Trophoblast glycoprotein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling 5T4 with purified ab134162 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134162).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placenta tissue labelling 5T4 with unpurified ab134162 at 1/100. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134162).

    • Immunohistochemical staining using ab134162 on paraffin embedded human endometrial cancer.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134162).

    • Immunohistochemical staining using ab134162 on paraffin embedded human placenta tissue.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134162).

    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134162).

    References

    ab201711 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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