RecombinantRabMAb

Recombinant Anti-5T4 antibody [EPR5530] (ab129058)

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Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
  • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
  • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
  • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
  • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
  • Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)
  • Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)
  • Anti-5T4 antibody [EPR5530] (ab129058)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5530] to 5T4
  • Suitable for: WB, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-5T4 antibody [EPR5530]
    See all 5T4 primary antibodies

  • Description

    Rabbit monoclonal [EPR5530] to 5T4

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human 5T4 aa 350-450 (intracellular). The exact sequence is proprietary.

  • Positive control

    • HT 1376, HeLa, JAR and MCF7 cell lysates; MCF7 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab129058 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 72-80 kDa (predicted molecular weight: 46 kDa).
IP 1/80.
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Tissue specificity

      Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.

    • Sequence similarities

      Contains 6 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.

    • Cellular localization

      Membrane.
    • Information by UniProt

    • Database links

      see all

    • Form

      5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1–60 (Ward et al., 2006).

    • Alternative names

      • 5T4 antibody
      • 5T4 oncofetal antigen antibody
      • 5T4 oncofetal trophoblast glycoprotein antibody
      • 5T4 oncotrophoblast glycoprotein antibody
      • 5T4AG antibody
      • AW495680 antibody
      • M6P1 antibody
      • TPBG antibody
      • TPBG_HUMAN antibody
      • Trophoblast glycoprotein antibody
      see all

    Images

    • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
      Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
      Anti-5T4 antibody [EPR5530] (ab129058) at 1/5000 dilution (purified) + Rat brain at 20 µg

      Secondary
      HRP goat-anti rabbit (H+L) at 1/1000 dilution

      Predicted band size: 46 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
      Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
      Anti-5T4 antibody [EPR5530] (ab129058) at 1/2000 dilution (unpurified) + Rat brain at 20 µg

      Secondary
      HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 46 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
      Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
      All lanes : Anti-5T4 antibody [EPR5530] (ab129058) at 1/5000 dilution (purified)

      Lane 1 : HT-1376 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 46 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
      Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
      All lanes : Anti-5T4 antibody [EPR5530] (ab129058) at 1/2000 dilution (unpurified)

      Lane 1 : HT-1376 cell lysate
      Lane 2 : HeLa cell lysate

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : HRP goat anti-rabbit (H+L) at 1/1000 dilution

      Predicted band size: 46 kDa



      Blocking buffer: 5% NFDM/TBST

      Dilution buffer: 5% NFDM/TBST

    • Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
      Western blot - Anti-5T4 antibody [EPR5530] (ab129058)
      All lanes : Anti-5T4 antibody [EPR5530] (ab129058) at 1/1000 dilution (unpurified)

      Lane 1 : HT 1376 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : JAR cell lysate
      Lane 4 : MCF7 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 46 kDa

    • Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)
      Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)

      ab129058 (unpurified) at 1/50 immunoprecipitating 5T4 in MCF-7 (Lane 1). For western blotting, a HRP goat anti-rabbit was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)
      Immunoprecipitation - Anti-5T4 antibody [EPR5530] (ab129058)

      ab129058 (unpurified) at 1/50 immunoprecipitating 5T4 in MCF-7 (Lane 1). For western blotting, a HRP goat anti-rabbit was used as the secondary antibody (1/1000).

      Blocking buffer and concentration: 5% NFDM/TBST.

      Diluting buffer and concentration: 5% NFDM /TBST.

    • Anti-5T4 antibody [EPR5530] (ab129058)
      Anti-5T4 antibody [EPR5530] (ab129058)

    References (1)

    Publishing research using ab129058? Please let us know so that we can cite the reference in this datasheet.

    ab129058 has been referenced in 1 publication.

    • Shor B  et al. Enhanced Antitumor Activity of an Anti-5T4 Antibody-Drug Conjugate in Combination with PI3K/mTOR inhibitors or Taxanes. Clin Cancer Res 22:383-94 (2016). WB ; Human . PubMed: 26319086

    Customer reviews and Q&As

    Show All Reviews Q&A
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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-5T4 antibody [EPR5530]

     Good
    Abreviews
    This product is known to not work in this application or species.
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (placenta)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH 9.0
    Permeabilization
    No
    Specification
    placenta
    Blocking step
    Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Dr. Lennart Olsson

    Verified customer

    Submitted Apr 25 2019

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