Recombinant
RabMAb

Recombinant Anti-5T4 antibody [EPR5530] - BSA and Azide free (ab189508)

Overview

  • Product name

    Anti-5T4 antibody [EPR5530] - BSA and Azide free
    See all 5T4 primary antibodies
  • Description

    Rabbit monoclonal [EPR5530] to 5T4 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IP, WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human 5T4 aa 350-450 (intracellular). The exact sequence is proprietary.

  • Positive control

    • HT 1376, HeLa, JAR and MCF7 cell lysates; MCF7 cells.
  • General notes

    Ab189508 is the carrier-free version of ab129058. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab189508 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.??

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab189508 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Detects a band of approximately 72-80 kDa (predicted molecular weight: 46 kDa).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Tissue specificity

      Expressed by all types of trophoblasts as early as 9 weeks of development. Specific for trophoblastic cells except for amniotic epithelium. In adult tissues, the expression is limited to a few epithelial cell types but is found on a variety of carcinoma.
    • Sequence similarities

      Contains 6 LRR (leucine-rich) repeats.
      Contains 1 LRRCT domain.
      Contains 1 LRRNT domain.
    • Cellular localization

      Membrane.
    • Information by UniProt
    • Database links

    • Form

      5T4 is a 72 kDa transmembrane glycoprotein that exhibits restricted expression in human and mouse adult tissues, is upregulated on many carcinomas and tumour expression correlates with poorer clinical outcome in ovarian, gastric and colorectal cancers. mES cells lack cell surface expression of the 5T4 antigen, and both protein and mRNA are rapidly upregulated following induction of differentiation. This proteins expression correlates with downregulation of OCT-4 and Tra-1–60 (Ward et al., 2006).
    • Alternative names

      • 5T4 antibody
      • 5T4 oncofetal antigen antibody
      • 5T4 oncofetal trophoblast glycoprotein antibody
      • 5T4 oncotrophoblast glycoprotein antibody
      • 5T4AG antibody
      • AW495680 antibody
      • M6P1 antibody
      • TPBG antibody
      • TPBG_HUMAN antibody
      • Trophoblast glycoprotein antibody
      see all

    References

    ab189508 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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