6-Carboxyfluorescein N-hydroxysuccinimide ester, Fluorescent labeling reagent (ab144945)

Overview

  • Product name

    6-Carboxyfluorescein N-hydroxysuccinimide ester, Fluorescent labeling reagent
  • Description

    Commonly used fluorescent reagent for labeling of peptides, proteins and nucleotides.
  • Alternative names

    • 1-[[3’,6’-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9’-[9H]xanthen]-6-yl]carbonyl]oxy]-2,5-pyrrolidinedione
    • 3’,6’-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9’-[9H]xanthene]-6-carboxylic Acid 2,5-Dioxo-1-pyrrolidinyl Ester
    • 6-Carboxyfluorescein-N-succinimidyl Ester
    • 6-Carboxyfluorescein-NHS
    • 6-FAM SE
    • Fluorescein-6-carboxylic Acid N-Succinimidyl Ester
    see all
  • Biological description

    Commonly used fluorescent reagent for labeling of peptides, proteins and nucleotides. Amine-reactive succinimidyl ester carboxyfluorescein which yields carboxyamides that are more resistant to hydrolysis than fluorescein conjugates prepared with FITC.

  • General notes

    Fluorescence:  λex 492 nm; λem 517 nm (pH 8.0).

  • CAS Number

    92557-81-8
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    492nm
  • Emission

    517nm
  • Chemical name

    6-Carboxyfluorescein N-hydroxysuccinimide ester
  • Molecular weight

    473.39
  • Molecular formula

    C25H15NO9
  • Storage instructions

    Store at Room Temperature. The product can be stored for up to 12 months.
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • Source

    Synthetic

References

ab144945 has not yet been referenced specifically in any publications.

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