6-CFDA N-succinimidyl ester, Fluorogenic esterase substrate (ab145296)

Overview

  • Product name

    6-CFDA N-succinimidyl ester, Fluorogenic esterase substrate
  • Description

    Cell-permeable, fluorogenic, non-selective esterase substrate
  • Biological description

    Cell-permeable, fluorogenic, non-selective esterase substrate. Hydrolyzes to fluorescent 6-carboxyfluorescein (ab145294) and covalently labels intracellular proteins. 6-CFDA (ab145298) derivative. 5-CFDA N-succinimidyl ester (ab145292) analog.

  • Purity

    > 90%
  • CAS Number

    150206-15-8
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    495nm
  • Emission

    520nm
  • Chemical name

    (2,5-Dioxopyrrolidin-1-yl) 3',6'-diacetyloxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylate
  • Molecular weight

    557.46
  • Molecular formula

    C29H19NO11
  • PubChem identifier

    5048409
  • Storage instructions

    Store at -20°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=C(C=CC(=C5)C(=O)ON6C(=O)CCC6=O)C(=O)O3
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Stich S  et al. Regenerative and immunogenic characteristics of cultured nucleus pulposus cells from human cervical intervertebral discs. PLoS One 10:e0126954 (2015). Read more (PubMed: 25993467) »
  • Yang T  et al. A novel nonradioactive CFDA assay to monitor the cellular immune response in myeloid leukemia. Immunobiology 218:548-53 (2013). Read more (PubMed: 22883564) »
  • Urbani S  et al. Use of CFDA-SE for evaluating the in vitro proliferation pattern of human mesenchymal stem cells. Cytotherapy 8:243-53 (2006). Read more (PubMed: 16793733) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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