6-FAM (6-Carboxyfluorescein), Amine-reactive fluorescent label (ab145294)

Overview

  • Product name

    6-FAM (6-Carboxyfluorescein), Amine-reactive fluorescent label
  • Description

    Amine-reactive fluorescent label
  • Biological description

    Amine-reactive fluorescent label. 5-FAM (ab145293) isomer. 6-CFDA (ab145298) and 6-CFDA N-succinimidyl ester (ab145296) hydrolytic product. Polar tracer. Covalently labels peptides and proteins. Deprotonates below pH 7 resulting in reduced fluorescence. Compatible with most fluorescence detection equipment.

  • Purity

    > 97%
  • CAS Number

    3301-79-9
  • Chemical structure

    Chemical Structure

Properties

  • Excitation

    495nm
  • Emission

    520nm
  • Chemical name

    3',6'-Dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid
  • Molecular weight

    376.32
  • Molecular formula

    C21H12O7
  • PubChem identifier

    76806
  • Storage instructions

    Store at +4°C. Store under desiccating conditions. The product can be stored for up to 12 months.
  • Solubility overview

    Soluble in DMSO
  • Handling

    Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.

    Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

  • SMILES

    C1=CC2=C(C=C1C(=O)O)C3(C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O)OC2=O
  • Source

    Synthetic

  • Research areas

References

This product has been referenced in:

  • Gholami Z  et al. PNA-induced assembly of fluorescent proteins using DNA as a framework. Bioconjug Chem 24:1378-86 (2013). Read more (PubMed: 23848261) »
  • Ban E  et al. Simultaneous detection of multiple microRNAs for expression profiles of microRNAs in lung cancer cell lines by capillary electrophoresis with dual laser-induced fluorescence. J Chromatogr A 1315:195-9 (2013). Read more (PubMed: 24094482) »
  • Strotbek M  et al. Stable microRNA expression enhances therapeutic antibody productivity of Chinese hamster ovary cells. Metab Eng 20:157-66 (2013). Read more (PubMed: 24144501) »

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES, NOT FOR USE IN HUMANS"
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