Key features and details
- Sensitivity: 1.4 pg/ml
- Range: 3.2 pg/ml - 50000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Saliva, Serum, Tissue Culture Media, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human, a wide range of other species
Product name6-keto-PGF1 alpha ELISA Kit
Intra-assay Sample n Mean SD CV% Low conc. 10 41pg/ml 13.1% Medium conc. 10 240pg/ml 4.8% High conc. 10 4886pg/ml 2.9% Inter-assay Sample n Mean SD CV% Low conc. 8 239pg/ml 7.9% Medium conc. 8 1220pg/ml 5.2% High conc. 8 4828pg/ml 6%
Sample typeCell culture supernatant, Saliva, Urine, Serum, Plasma, Tissue Culture Media
Range3.2 pg/ml - 50000 pg/ml
Sample specific recovery Sample type Average % Range Saliva 110.7 % - % Urine 108.7 % - % Serum 108.4 % - % Tissue Culture Media 106.7 % - %
Assay time3h 00m
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human, a wide range of other species
Abcam’s 6-keto-PGF1 alpha in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of 6-keto-PGF1 alpha in serum, urine, tissue culture media and other biological fluids.
A donkey anti-sheep IgG antibody has been precoated onto 96-well plates. Standards or test samples are added to the wells, along with an alkaline phosphatase (AP) conjugated-6-keto-PGF1 alpha antigen and a polyclonal sheep antibody specific to 6-keto-PGF1 alpha. After incubation the excess reagents are washed away. pNpp substrate is added. After a short incubation the enzyme reaction is stopped and the yellow color generated by alkaline phosphatase is read at 405 nm. The intensity of the yellow coloration is inversely proportional to the amount of 6-keto-PGF1α captured in the plate.
6-keto-PGF1α is a stable hydrolysis product of prostacyclin (PGI2). Due to the short half-life of PGI2 in buffer (2-3 minutes) the production of prostacyclin is typically monitored by measurement of the 6-keto-PGF1α metabolite and its derivative in urine, 2,3-dinor-6-keto-PGF1α. PGI2 has autocoid functions that include platelet aggregation, inhibition of vasoconstrictor responses to pressor hormones, platelet anti- and disaggregatory properties, and reproductive functions. The effects are induced by receptor-mediated elevation of cAMP. Prostacyclin has clinical applications in atherosclerosis and protection from myocardial ischemia.
Compound Cross Reactivity
3.17% PGF2α 1.67% PGD2 0.60% PGF1α 0.60% PGE1 0.20% 6,15-diketo-13,14-dihydro-PGF1α 0.12% 13,14-dihydro-15-keto-PGF1α <0.01% 15-keto-PGF2α <0.01% PGA2 <0.01% PGB1 <0.01% PGE2 <0.01% Thromboxane B2 <0.01% 15-HpETE <0.01% 2-Arachidonoylglycerol <0.01% Anandamide <0.01%
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer Concentrate 1 x 27ml 6-keto-PGF1 alpha Alkaline Phosphatase Conjugate 1 x 5ml 6-keto-PGF1 alpha EIA Antibody 1 x 5ml 6-keto-PGF1 alpha Standard 1 x 500µl Assay Buffer 1 x 27ml Donkey anti-sheep IgG Microplate (12 x 8 wells) 1 unit Plate Sealer 1 unit pNpp Substrate 1 x 20ml Stop Solution 1 x 5ml
- 6-keto Prostaglandin F1a
ab133023 has been referenced in 1 publication.
- Wang F et al. Antithrombin III/SerpinC1 insufficiency exacerbates renal ischemia/reperfusion injury. Kidney Int 88:796-803 (2015). PubMed: 26108065