Product nameAnti-6-Nitrotryptophan antibody [2D12]
See all 6-Nitrotryptophan primary antibodies
DescriptionMouse monoclonal [2D12] to 6-Nitrotryptophan
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to 6-Nitrotryptophan conjugated to keyhole limpet haemocyanin.
- WB: 6-Nitrotryptophan-BSA. Flow cyt: SH-SY5Y cells (treated with hydrogen peroxide).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab243065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
All lanes : Anti-6-Nitrotryptophan antibody [2D12] (ab243065) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : BSA at 1 µg
Lane 3 : 6-Nitrotryptophan-BSA at 0.5 µg
Lane 4 : 6-Nitrotryptophan-BSA at 1 µg
Lane 5 : 7-Ketocholesterol-BSA at 0.5 µg
Lane 6 : 7-Ketocholesterol-BSA at 1 µg
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Exposure time: 1 minute
Primary incubation for 2 hours at room temperature.
Secondary incubation for 1 hr at RT.
Block: 5% Skimmed milk in TBST.
Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (+/- 250 μM hydrogen peroxide treatment for 24 hours) labeling 6-Nitrotryptophan with ab243065 at 1/50 dilution for 30 minutes on ice compared with a Isotype control IgG and an unlabeled control. A Goat Anti-Mouse: PE at 1/100 dilution was used as the secondary antibody.
ab243065 has not yet been referenced specifically in any publications.