Key features and details
- Mouse monoclonal [2D12] to 6-Nitrotryptophan
- Suitable for: WB, Flow Cyt
- Reacts with: Species independent
- Isotype: IgG1
Product nameAnti-6-Nitrotryptophan antibody [2D12]
See all 6-Nitrotryptophan primary antibodies
DescriptionMouse monoclonal [2D12] to 6-Nitrotryptophan
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Species independent
Chemical/ Small Molecule corresponding to 6-Nitrotryptophan conjugated to keyhole limpet haemocyanin.
- WB: 6-Nitrotryptophan-BSA. Flow cyt: SH-SY5Y cells (treated with hydrogen peroxide).
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityProtein G purified
Purification notesPurified from TCS.
Our Abpromise guarantee covers the use of ab243065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
All lanes : Anti-6-Nitrotryptophan antibody [2D12] (ab243065) at 1/1000 dilution
Lane 1 : BSA at 0.5 µg
Lane 2 : BSA at 1 µg
Lane 3 : 6-Nitrotryptophan-BSA at 0.5 µg
Lane 4 : 6-Nitrotryptophan-BSA at 1 µg
Lane 5 : 7-Ketocholesterol-BSA at 0.5 µg
Lane 6 : 7-Ketocholesterol-BSA at 1 µg
All lanes : Goat Anti-Mouse IgG: HRP at 1/2000 dilution
Exposure time: 1 minute
Primary incubation for 2 hours at room temperature.
Secondary incubation for 1 hr at RT.
Block: 5% Skimmed milk in TBST.
Flow cytometric analysis of 90% methanol-fixed SH-SY5Y (human neuroblastoma cell line from bone marrow) cells (+/- 250 μM hydrogen peroxide treatment for 24 hours) labeling 6-Nitrotryptophan with ab243065 at 1/50 dilution for 30 minutes on ice compared with a Isotype control IgG and an unlabeled control. A Goat Anti-Mouse: PE at 1/100 dilution was used as the secondary antibody.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab243065 has not yet been referenced specifically in any publications.