Overview

  • Product name
    6-Phosphofructokinase Activity Assay Kit (Colorimetric)
  • Detection method
    Colorimetric
  • Sample type
    Cell Lysate, Tissue Lysate
  • Assay type
    Enzyme activity
  • Sensitivity
    < 1 mU/well
  • Range
    2 nmol/well - 10 nmol/well
  • Assay time
    1h 30m
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    6-Phosphofructokinase Activity Assay kit ab155898 is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.


    In the PFK activity assay protocol, PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.


    PFK activity assay protocol summary:
    - add samples and standards to wells
    - add reaction mix
    - analyze with a microplate reader over 20-60 min

  • Notes

    Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet).

  • Platform
    Microplate reader

Properties

Images

  • PFK activity determined in bovine liver and HeLa cell lysate. Assay performed following kit protocol.

Protocols

References

ab155898 has not yet been referenced specifically in any publications.

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