Name: 6-Phosphofructokinase Activity Assay Kit (Colorimetric)
SKU: ab155898

Product name

6-Phosphofructokinase Activity Assay Kit (Colorimetric)
Detection method

Colorimetric
Sample type

Cell Lysate, Tissue Lysate
Assay type

Enzyme activity
Sensitivity

< 1 mU/well
Range

2 nmol/well - 10 nmol/well
Assay time

1h 30m
Species reactivity

Reacts with: Other species, Mammals
Product overview

6-Phosphofructokinase Activity Assay kit ab155898 is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.

In the PFK activity assay protocol, PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.

PFK activity assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader over 20-60 min
Notes

Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet).
Platform

Microplate reader

Phosphofructokinase activity using ab155898

PFK activity determined in bovine liver and HeLa cell lysate. Assay performed following kit protocol.

ab155898 has not yet been referenced specifically in any publications.

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Customer reviews and Q&As

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

6-Phosphofructokinase Activity Assay Kit (Colorimetric) (ab155898)