6-Phosphofructokinase Activity Assay Kit (Colorimetric) (ab155898)
- Datasheet
- References (2)
- Protocols
Overview
-
Product name
6-Phosphofructokinase Activity Assay Kit (Colorimetric) -
Detection method
Colorimetric -
Sample type
Cell Lysate, Tissue Lysate -
Assay type
Enzyme activity -
Sensitivity
< 1 mU/well -
Range
2 nmol/well - 10 nmol/well -
Assay time
1h 30m -
Species reactivity
Reacts with: Other species, Mammals -
Product overview
6-Phosphofructokinase Activity Assay kit ab155898 is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.
In the PFK activity assay protocol, PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.
PFK activity assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader over 20-60 min -
Notes
Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet).
-
Platform
Microplate reader
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests ATP Orange 1 vial NADH Standard Yellow 1 vial PFK Assay Buffer WM 1 x 27ml PFK Developer Red 1 vial PFK Enzyme Mix Green 1 vial PFK Positive Control Purple 1 vial PFK Substrate Blue 1 vial -
Research areas
Images
-
Phosphofructokinase activity using ab155898PFK activity determined in bovine liver and HeLa cell lysate. Assay performed following kit protocol.
Protocols
References
This product has been referenced in:
- Caldwell CC et al. Treadmill exercise rescues mitochondrial function and motor behavior in the CAG140 knock-in mouse model of Huntington's disease. Chem Biol Interact 315:108907 (2019). Read more (PubMed: 31778667) »
- Schoenwaelder SM et al. 14-3-3? regulates the mitochondrial respiratory reserve linked to platelet phosphatidylserine exposure and procoagulant function. Nat Commun 7:12862 (2016). Read more (PubMed: 27670677) »
