6-Phosphofructokinase Activity Assay Kit (Colorimetric) (ab155898)
- Datasheet
- References
- Protocols
Overview
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Product name6-Phosphofructokinase Activity Assay Kit (Colorimetric)
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Detection methodColorimetric
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Sample typeCell Lysate, Tissue Lysate
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Assay typeEnzyme activity
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Sensitivity< 1 mU/well
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Range2 nmol/well - 10 nmol/well
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Assay time1h 30m
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Species reactivityReacts with: Other species, Mammals
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Product overview
6-Phosphofructokinase Activity Assay kit ab155898 is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.
In the PFK activity assay protocol, PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.
PFK activity assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader over 20-60 min -
Notes
Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet).
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PlatformMicroplate reader
Properties
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Storage instructionsStore at -20°C. Please refer to protocols.
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Components Identifier 100 tests ATP Orange 1 vial NADH Standard Yellow 1 vial PFK Assay Buffer WM 1 x 27ml PFK Developer Red 1 vial PFK Enzyme Mix Green 1 vial PFK Positive Control Purple 1 vial PFK Substrate Blue 1 vial -
Research areas
Images
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Phosphofructokinase activity using ab155898PFK activity determined in bovine liver and HeLa cell lysate. Assay performed following kit protocol.
Protocols
References
ab155898 has not yet been referenced specifically in any publications.
