6-Phosphofructokinase Activity Assay Kit (Colorimetric) (ab155898)

Overview

  • Product name
    6-Phosphofructokinase Activity Assay Kit (Colorimetric)
  • Sample type
    Cell Lysate, Tissue Lysate
  • Assay type
    Enzyme activity
  • Sensitivity
    < 1 mU/well
  • Range
    2 nmol/well - 10 nmol/well
  • Assay time
    1h 30m
  • Species reactivity
    Reacts with: Other species, Mammals
  • Product overview

    In Abcam's 6-Phosphofructokinase Activity Assay kit (ab155898), PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm. PFK activity assay is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.

  • Notes

    Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet). In humans, PFK deficiency causes glycogen storage disease, also called Tarui’s disease, which is characterized by exercise-induced muscle weakness and cramps. On the other hand, increased PFK activity contributes to cancer cell proliferation and tumorigenicity. Early detection of abnormal phosphofructokinase activity is crucial for diagnosis, prediction and therapeutic strategy.

Properties

Images

  • PFK activity determined in bovine liver and HeLa cell lysate. Assay performed following kit protocol.

Protocols

References

ab155898 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab155898.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up