Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Assay time: 1 hr 30 min
- Sample type: Cell Lysate, Tissue Lysate
- Sensitivity: 1 mU/well
Product name6-Phosphofructokinase Activity Assay Kit (Colorimetric)
Sample typeCell Lysate, Tissue Lysate
Assay typeEnzyme activity
Sensitivity< 1 mU/well
Range2 nmol/well - 10 nmol/well
Assay time1h 30m
Species reactivityReacts with: Mammals, Other species
6-Phosphofructokinase Activity Assay kit ab155898 is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.
In the PFK activity assay protocol, PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.
PFK activity assay protocol summary:
- add samples and standards to wells
- add reaction mix
- analyze with a microplate reader over 20-60 min
This product is manufactured by BioVision, an Abcam company and was previously called K776 Phosphofructokinase (PFK) Activity Colorimetric Assay Kit. K776-100 is the same size as the 100 test size of ab155898.
Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet).
Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 100 tests ATP Orange 1 vial NADH Standard Yellow 1 vial PFK Assay Buffer WM 1 x 27ml PFK Developer Red 1 vial PFK Enzyme Mix Green 1 vial PFK Positive Control Purple 1 vial PFK Substrate Blue 1 vial
ab155898 has been referenced in 4 publications.
- Park JS et al. Mechanical regulation of glycolysis via cytoskeleton architecture. Nature 578:621-626 (2020). PubMed: 32051585
- Caldwell CC et al. Treadmill exercise rescues mitochondrial function and motor behavior in the CAG140 knock-in mouse model of Huntington's disease. Chem Biol Interact 315:108907 (2019). PubMed: 31778667
- Fujiwara H et al. Mutant IDH1 confers resistance to energy stress in normal biliary cells through PFKP-induced aerobic glycolysis and AMPK activation. Sci Rep 9:18859 (2019). PubMed: 31827136
- Schoenwaelder SM et al. 14-3-3? regulates the mitochondrial respiratory reserve linked to platelet phosphatidylserine exposure and procoagulant function. Nat Commun 7:12862 (2016). PubMed: 27670677