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6-Phosphofructokinase Activity Assay Kit (Colorimetric) (ab155898)

  • Datasheet
  • SDS
  • Protocol Booklet
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Phosphofructokinase activity using ab155898

    Key features and details

    • Assay type: Enzyme activity
    • Detection method: Colorimetric
    • Platform: Microplate reader
    • Assay time: 1 hr 30 min
    • Sample type: Cell Lysate, Tissue Lysate
    • Sensitivity: 1 mU/well

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    Overview

    • Product name

      6-Phosphofructokinase Activity Assay Kit (Colorimetric)
    • Detection method

      Colorimetric
    • Sample type

      Cell Lysate, Tissue Lysate
    • Assay type

      Enzyme activity
    • Sensitivity

      < 1 mU/well
    • Range

      2 nmol/well - 10 nmol/well
    • Assay time

      1h 30m
    • Species reactivity

      Reacts with: Mammals, Other species
    • Product overview

      6-Phosphofructokinase Activity Assay kit ab155898 is simple, robust, and rapid and can detect phosphofructokinase activity less than 1 mU.


      In the PFK activity assay protocol, PFK (phosphofructokinase) converts fructose-6-phosphate and ATP to fructose-diphosphate and ADP. The ADP in the presence of substrate and enzyme mix is converted to AMP and NADH, which reduces a colorless probe to a colored product with strong absorbance at 450 nm.


      PFK activity assay protocol summary:
      - add samples and standards to wells
      - add reaction mix
      - analyze with a microplate reader over 20-60 min

    • Notes

      Phosphofructokinase (PFK) is a key glycolytic enzyme and plays a major regulatory role during glycolysis. This enzyme is present in bacteria, plants and animals. There are 2 types of PFKs - PFK1 and PFK2. In the presence of ATP, PFK1 & PFK2 catalyzes the conversion of fructose-6-phosphate to fructose-1,6-diphosphate and fructose-2,6-diphosphate respectively and ADP. PFK has 3 major isoforms in mammals: PFK-M (muscle), PFK-L (liver) and PFK-P (platelet).

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    • Platform

      Microplate reader

    Properties

    • Storage instructions

      Store at -20°C. Please refer to protocols.
    • Components Identifier 100 tests
      ATP Orange 1 vial
      NADH Standard Yellow 1 vial
      PFK Assay Buffer WM 1 x 27ml
      PFK Developer Red 1 vial
      PFK Enzyme Mix Green 1 vial
      PFK Positive Control Purple 1 vial
      PFK Substrate Blue 1 vial
    • Research areas

      • Signal Transduction
      • Metabolism
      • Energy Metabolism
      • Kits/ Lysates/ Other
      • Kits
      • Cell Metabolism Kits
      • Sugar Assays
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Metabolism of carbohydrates
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Carbohydrate metabolism
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Energy transfer pathways
      • Energy Metabolism

    Associated products

      Images

      • Phosphofructokinase activity using ab155898
        Phosphofructokinase activity using ab155898
        PFK activity determined in bovine liver and HeLa cell lysate. Assay performed following kit protocol.

      Protocols

      • Protocol Booklet

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (3)

      Publishing research using ab155898? Please let us know so that we can cite the reference in this datasheet.

      ab155898 has been referenced in 3 publications.

      • Caldwell CC  et al. Treadmill exercise rescues mitochondrial function and motor behavior in the CAG140 knock-in mouse model of Huntington's disease. Chem Biol Interact 315:108907 (2019). PubMed: 31778667
      • Fujiwara H  et al. Mutant IDH1 confers resistance to energy stress in normal biliary cells through PFKP-induced aerobic glycolysis and AMPK activation. Sci Rep 9:18859 (2019). PubMed: 31827136
      • Schoenwaelder SM  et al. 14-3-3? regulates the mitochondrial respiratory reserve linked to platelet phosphatidylserine exposure and procoagulant function. Nat Commun 7:12862 (2016). PubMed: 27670677

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