650 nm Stop Solution for TMB Substrate (ab171531)

Overview

  • Product name
    650 nm Stop Solution for TMB Substrate
    See all ELISA stop solution reagents
  • Description
    650 nm Stop Solution for TMB Substrate
  • Tested applications
    Suitable for: ELISAmore details
    Unsuitable for: IHC-Fr or IHC-P
  • General notes

    Abcam’s 650 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection. The acidic formulation is a proprietary solution used to stop TMB ELISA Substrate reactions (ab171522, ab171523, ab171524, ab171525, ab171526 and ab171527) in the blue range of 620 nm to 650 nm.

    Product Use:
    Equal volumes of TMB ELISA Substrate and 650 nm Stop Solution for TMB Substrate should be used.

    Storage Instructions:

    Product should be stored at 2-8°C. Exposure to direct sunlight and other UV sources should be avoided due to the light sensitive nature of the TMB molecule.

Properties

  • Form
    Liquid
  • Storage instructions
    Store at +4°C.
  • Storage buffer
    Constituent: 2% Proprietary Component 3
  • Concentration information loading...

Applications

Our Abpromise guarantee covers the use of ab171531 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 650 nm Stop Solution for TMB Substrate should be used.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.
  • References

    ab171531 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    The lab that provides the 650nm TMB stop solution to us sent data that shows no difference in detection limit compared to the standard 450nm stop solution. The 650nm actually had the advantage of increased dynamic range. In their words: "The yellow product has a higher extinction coefficient that yields about 2.6 times the absorbance signal for a given concentration of analyte. There is an ongoing perception that converting the TMB to the yellow product increases the ability to detect the analyte... Addition of the yellow stop clearly produced higher signals at each analyte concentration thereby showing an increased sensitivity; however, some points were saturated at an OD ≥ 4.0. A benefit of reading at 650 nm is that these points now fall in the quantifiable range....The increase in standard deviation for each point is about 2.6-fold higher for the 450 nm reading versus the 650 nm reading. This is equivalent to the signal increase achieved by stopping and reading at 450 nm. So, the signal and the error have been increased by exactly the same factor. This results in no net change in the detection limit....There was no difference in the detection limit or the ability to distinguish between msIgG concentrations across this experiment. Stopping the TMB reaction in the blue and reading at 650 nm is a valuable strategy to increase the dynamic range of an assay without sacrificing the detection."

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    Question
    Answer

    The pH is 7.5 - 11.5

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    Question
    Answer

    Refrigerated temperatures will not harm the reagent.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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