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    650-nm-stop-solution-for-tmb-substrate-ab171531.pdf

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650 nm Stop Solution for TMB Substrate (ab171531)

  • Datasheet
  • SDS
Submit a review Q&A (3)

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ELISA
Product image
TMB ELISA Substrate (High Sensitivity) (ab171523)

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Overview

  • Product name

    650 nm Stop Solution for TMB Substrate
    See all ELISA stop solution reagents
  • Description

    650 nm Stop Solution for TMB Substrate
  • Tested applications

    Suitable for: ELISAmore details
    Unsuitable for: IHC-Fr or IHC-P
  • General notes

    Abcam’s 650 nm Stop Solution for TMB Substrate is a ready-to-use solution for colorimetric HRP-based ELISA detection. The acidic formulation is a proprietary solution used to stop TMB ELISA Substrate reactions (ab171522, ab171523, ab171524, ab171525, ab171526 and ab171527) in the blue range of 620 nm to 650 nm.

    Product Use:
    Equal volumes of TMB ELISA Substrate and 650 nm Stop Solution for TMB Substrate should be used.

    Storage Instructions:

    Product should be stored at 2-8°C. Exposure to direct sunlight and other UV sources should be avoided due to the light sensitive nature of the TMB molecule.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at Room Temperature. Store at +4°C.
  • Storage buffer

    pH: 8
  • Concentration information loading...

Associated products

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab171531 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    ELISA
    Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 650 nm Stop Solution for TMB Substrate should be used.
    Notes
    ELISA
    Use at an assay dependent concentration. Equal volumes of TMB ELISA Substrate and 650 nm Stop Solution for TMB Substrate should be used.
  • Application notes
    Is unsuitable for IHC-Fr or IHC-P.
  • Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab171531? Please let us know so that we can cite the reference in this datasheet.

    ab171531 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a question

    1-3 of 3 Q&A

    Question

    What is the disadvantage, if any, of the 650 nm TMB stop solution compared to the 450 nm stop solution? The 450 nm stop is more common.

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    Abcam community

    Verified customer

    Asked on Jun 02 2014

    Answer

    The lab that provides the 650nm TMB stop solution to us sent data that shows no difference in detection limit compared to the standard 450nm stop solution. The 650nm actually had the advantage of increased dynamic range. In their words: "The yellow product has a higher extinction coefficient that yields about 2.6 times the absorbance signal for a given concentration of analyte. There is an ongoing perception that converting the TMB to the yellow product increases the ability to detect the analyte... Addition of the yellow stop clearly produced higher signals at each analyte concentration thereby showing an increased sensitivity; however, some points were saturated at an OD ≥ 4.0. A benefit of reading at 650 nm is that these points now fall in the quantifiable range....The increase in standard deviation for each point is about 2.6-fold higher for the 450 nm reading versus the 650 nm reading. This is equivalent to the signal increase achieved by stopping and reading at 450 nm. So, the signal and the error have been increased by exactly the same factor. This results in no net change in the detection limit....There was no difference in the detection limit or the ability to distinguish between msIgG concentrations across this experiment. Stopping the TMB reaction in the blue and reading at 650 nm is a valuable strategy to increase the dynamic range of an assay without sacrificing the detection."

    Read More

    Tom Ruyle

    Abcam Scientific Support

    Answered on Jun 02 2014

    Question

    What is the pH of ab171531?

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    Abcam community

    Verified customer

    Asked on May 14 2013

    Answer

    The pH is 7.5 - 11.5

    Read More

    Abcam Scientific Support

    Answered on May 14 2013

    Question

    Will storing 650 nm Stop Solution for TMB Substrate at 2oC to 8oC harm the product?

    Read More

    Abcam community

    Verified customer

    Asked on May 14 2013

    Answer

    Refrigerated temperatures will not harm the reagent.

    Read More

    Abcam Scientific Support

    Answered on May 14 2013

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