Overview

  • Product name
    Anti-67kDa Laminin Receptor antibody [EPR8468]
    See all 67kDa Laminin Receptor primary antibodies
  • Description
    Rabbit monoclonal [EPR8468] to 67kDa Laminin Receptor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, Flow Cytmore details
    Unsuitable for: ICC or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human 67kDa Laminin Receptor (UniProt ID: P08865)

  • Positive control
    • WB: Lysates of K562, HeLa, HepG2, C6, RAW 264.7, PC 12, NIH/3T3 IHC-P: Human brain tissue, Human kidney tissue
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab133775 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 43 kDa (predicted molecular weight: 33 kDa).
IHC-P 1/250 - 1/500.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC or IP.
  • Target

    • Function
      Required for the assembly and/or stability of the 40S ribosomal subunit. Required for the processing of the 20S rRNA-precursor to mature 18S rRNA in a late step of the maturation of 40S ribosomal subunits. Also functions as a cell surface receptor for laminin. Plays a role in cell adhesion to the basement membrane and in the consequent activation of signaling transduction pathways. May play a role in cell fate determination and tissue morphogenesis. Acts as a PPP1R16B-dependent substrate of PPP1CA. Also acts as a receptor for several other ligands, including the pathogenic prion protein, viruses, and bacteria.
    • Sequence similarities
      Belongs to the ribosomal protein S2P family.
    • Post-translational
      modifications
      Acylated. Acylation may be a prerequisite for conversion of the monomeric 37 kDa laminin receptor precursor (37LRP) to the mature dimeric 67 kDa laminin receptor (67LR), and may provide a mechanism for membrane association.
      Cleaved by stromelysin-3 (ST3) at the cell surface. Cleavage by stromelysin-3 may be a mechanism to alter cell-extracellular matrix interactions.
    • Cellular localization
      Cell membrane. Cytoplasm. Nucleus. 67LR is found at the surface of the plasma membrane, with its C-terminal laminin-binding domain accessible to extracellular ligands. 37LRP is found at the cell surface, in the cytoplasm and in the nucleus (By similarity). Co-localizes with PPP1R16B in the cell membrane.
    • Information by UniProt
    • Database links
    • Alternative names
      • 34/67 kDa laminin receptor antibody
      • 37 kDa laminin receptor precursor antibody
      • 37/67 kDa laminin receptor antibody
      • 37LRP antibody
      • 40S ribosomal protein SA antibody
      • 67 kDa laminin receptor antibody
      • 67LR antibody
      • Colon carcinoma laminin binding protein antibody
      • Colon carcinoma laminin-binding protein antibody
      • LAMBR antibody
      • Laminin receptor 1 antibody
      • Laminin-binding protein precursor p40 antibody
      • LAMR 1 antibody
      • LamR antibody
      • LAMR1 antibody
      • LBP antibody
      • LBP/p40 antibody
      • LRP antibody
      • LRP/LR antibody
      • Multidrug resistance associated protein MGr1 Ag antibody
      • Multidrug resistance associated protein MGr1Ag antibody
      • Multidrug resistance-associated protein MGr1-Ag antibody
      • NEM/1CHD4 antibody
      • p40 antibody
      • Ribosomal Protein SA antibody
      • rpsA antibody
      • RSSA_HUMAN antibody
      • SA antibody
      see all

    Images

    • All lanes : Anti-67kDa Laminin Receptor antibody [EPR8468] (ab133775) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : C6 cell lysate
      Lane 5 : RAW 264.7 cell lysate
      Lane 6 : PC12 cell lysate
      Lane 7 : NIH/3T3 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 33 kDa
      Observed band size: 43 kDa
      why is the actual band size different from the predicted?

    • ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human brain tissue using immunohistochemical analysis.
    • ab133775, at 1/250, staining 67kDa Laminin Receptor in paraffin-embedded Human kidney tissue using immunohistochemical analysis.
    • Equilibrium disassociation constant (KD)
      Learn more about KD

      Click here to learn more about KD

    References

    ab133775 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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